A fluorescent hormone biosensor reveals the dynamics of jasmonate signalling in plants

Larrieu, Antoine; Champion, Antony; Legrand, Jonathan; Lavenus, Julien; Mast, David; Brunoud, Geraldine; Oh, Jaesung; Guyomarc�h, Soazig; Pizot, Maxime; Farmer, Edward E.; Turnbull, Colin; Vernoux, Teva; Bennett, Malcolm J.; Laplaze, Laurent

Abstract

Activated forms of jasmonic acid (JA) are central signals coordinating plant responses to stresses, yet tools to analyse their spatial and temporal distribution are lacking. Here we describe a JA perception biosensor termed Jas9-VENUS that allows the quantification of dynamic changes in JA distribution in response to stress with high spatiotemporal sensitivity. We show that Jas9-VENUS abundance is dependent on bioactive JA isoforms, the COI1 co-receptor, a functional Jas motif and proteasome activity. We demonstrate the utility of Jas9-VENUS to analyse responses to JA in planta at a cellular scale, both quantitatively and dynamically. This included using Jas9-VENUS to determine the cotyledon-to-root JA signal velocities on wounding, revealing two distinct phases of JA activity in the root. Our results demonstrate the value of developing quantitative sensors such as Jas9-VENUS to provide high-resolution spatiotemporal data about hormone distribution in response to plant abiotic and biotic stresses.

Citation

Larrieu, A., Champion, A., Legrand, J., Lavenus, J., Mast, D., Brunoud, G., …Laplaze, L. (2015). A fluorescent hormone biosensor reveals the dynamics of jasmonate signalling in plants. Nature Communications, 6(1), https://doi.org/10.1038/ncomms7043

• Journal Article Type: Article
• Acceptance Date: Dec 5, 2014
• Publication Date: Jan 1, 2015
• Deposit Date: Jul 14, 2015
• Publicly Available Date: Jul 14, 2015
• Journal: Nature Communications
• Electronic ISSN: 2041-1723
• Publisher: Nature Publishing Group
• Peer Reviewed: Peer Reviewed
• Volume: 6
• Issue: 1
• DOI: https://doi.org/10.1038/ncomms7043
• Keywords: Arabidopsis, jasmonate, jasminic acid, biosensor, signalling
• Public URL: https://nottingham-repository.worktribe.com/output/743032
• Publisher URL: http://www.nature.com/ncomms/2015/150116/ncomms7043/full/ncomms7043.html
• Related Public URLs: https://www.cpib.ac.uk/people/malcolm-bennett/