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Two zinc finger proteins with functions in m6A writing interact with HAKAI

Zhang, Mi; Bodi, Zsuzsanna; Mackinnon, Katarzyna; Zhong, Silin; Archer, Nathan; Mongan, Nigel P.; Simpson, Gordon G.; Fray, Rupert G.

Two zinc finger proteins with functions in m6A writing interact with HAKAI Thumbnail


Research Fellow

Zsuzsanna Bodi

Katarzyna Mackinnon

Silin Zhong

Professor of Oncology

Gordon G. Simpson

Professor of Epitranscriptomics


The methyltransferase complex (m6A writer), which catalyzes the deposition of N6-methyladenosine (m6A) in mRNAs, is highly conserved across most eukaryotic organisms, but its components and interactions between them are still far from fully understood. Here, using in vivo interaction proteomics, two HAKAI-interacting zinc finger proteins, HIZ1 and HIZ2, are discovered as components of the Arabidopsis m6A writer complex. HAKAI is required for the interaction between HIZ1 and MTA (mRNA adenosine methylase A). Whilst HIZ1 knockout plants have normal levels of m6A, plants in which it is overexpressed show reduced methylation and decreased lateral root formation. Mutant plants lacking HIZ2 are viable but have an 85% reduction in m6A abundance and show severe developmental defects. Our findings suggest that HIZ2 is likely the plant equivalent of ZC3H13 (Flacc) of the metazoan m6A-METTL Associated Complex.

Journal Article Type Article
Acceptance Date Feb 9, 2022
Online Publication Date Mar 2, 2022
Publication Date Mar 2, 2022
Deposit Date Feb 9, 2022
Publicly Available Date Mar 2, 2022
Journal Nature Communications
Electronic ISSN 2041-1723
Peer Reviewed Peer Reviewed
Volume 13
Issue 1
Article Number 1127
Public URL
Publisher URL


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