Nuclear Transfer in Ruminants

Lee, Joon Hee; Maalouf, Walid E.

Abstract

© Springer Science+Business Media New York 2015. Ruminants were the first mammalian species to be cloned successfully by nuclear transplantation. Those experiments were designed to multiply high merit animals (Willadsen, Nature 320(6057):63–65, 1986; Prather et al., Biol Reprod 37(4):859–866, 1987; Wilmut et al., Nature 385(6619):810–813, 1997). Since then, cloning has provided us with a vast amount of knowledge and information on the reprogramming ability of somatic cells to different cell types which became an important basis for stem cell research and human medicine. Nowadays, the goals of most nuclear transfer work vary widely but in most cases the micromanipulation procedures remain the same. However, differences between species require different technical considerations. In this chapter, we describe in detail somatic cell nuclear transfer which is the foremost method for cloning ruminants with specific reference to sheep and cattle.

Citation

Lee, J. H., & Maalouf, W. E. (2014). Nuclear Transfer in Ruminants. In N. Beaujean, H. Jammes, & A. Jouneau (Eds.), Nuclear reprogramming: methods and protocols (25-36). Springer New York. https://doi.org/10.1007/978-1-4939-1594-1_3

• Acceptance Date: Jul 26, 2014
• Online Publication Date: Sep 20, 2014
• Publication Date: Sep 20, 2014
• Deposit Date: Feb 7, 2017
• Publicly Available Date: Feb 7, 2017
• Electronic ISSN: 1064-3745
• Peer Reviewed: Peer Reviewed
• Issue: 1222
• Pages: 25-36
• Series Title: Methods in molecular biology
• Series Number: 1222
• Book Title: Nuclear reprogramming: methods and protocols
• ISBN: 9781493915934; 9781493915941
• DOI: https://doi.org/10.1007/978-1-4939-1594-1_3
• Public URL: https://nottingham-repository.worktribe.com/output/738514
• Publisher URL: http://dx.doi.org/10.1007/978-1-4939-1594-1_3
• Contract Date: Feb 7, 2017