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Variation in sulfur and selenium accumulation is controlled by naturally occurring isoforms of the key sulfur assimilation enzyme ADENOSINE 5′-PHOSPHOSULFATE REDUCTASE2 across the arabidopsis species range

Chao, Dai-Yin; Baraniecka, Patrycja; Danku, John; Koprivova, Anna; Lahner, Brett; Luo, Hongbong; Yakubova, Elena; Dilkes, Brian; Kopriva, Stanislav; Salt, David E.

Authors

Dai-Yin Chao

Patrycja Baraniecka

John Danku

Anna Koprivova

Brett Lahner

Hongbong Luo

Elena Yakubova

Brian Dilkes

Stanislav Kopriva

David E. Salt



Abstract

Natural variation allows the investigation of both the fundamental functions of genes and their role in local adaptation. As one of the essential macronutrients, sulfur is vital for plant growth and development and also for crop yield and quality. Selenium and sulfur are assimilated by the same process, and although plants do not require selenium, plant-based selenium is an important source of this essential element for animals. Here, we report the use of linkage mapping in synthetic F2 populations and complementation to investigate the genetic architecture of variation in total leaf sulfur and selenium concentrations in a diverse set of Arabidopsis (Arabidopsis thaliana) accessions. We identify in accessions collected from Sweden and the Czech Republic two variants of the enzyme ADENOSINE 5′-PHOSPHOSULFATE REDUCTASE2 (APR2) with strongly diminished catalytic capacity. APR2 is a key enzyme in both sulfate and selenate reduction, and its reduced activity in the loss-of-function allele apr2-1 and the two Arabidopsis accessions Hodonín and Shahdara leads to a lowering of sulfur flux from sulfate into the reduced sulfur compounds, cysteine and glutathione, and into proteins, concomitant with an increase in the accumulation of sulfate in leaves. We conclude from our observation, and the previously identified weak allele of APR2 from the Shahdara accession collected in Tadjikistan, that the catalytic capacity of APR2 varies by 4 orders of magnitude across the Arabidopsis species range, driving significant differences in sulfur and selenium metabolism. The selective benefit, if any, of this large variation remains to be explored.

Journal Article Type Article
Publication Date Sep 22, 2014
Journal Plant Physiology
Print ISSN 0032-0889
Electronic ISSN 1532-2548
Publisher American Society of Plant Biologists
Peer Reviewed Peer Reviewed
Volume 166
Issue 3
APA6 Citation Chao, D., Baraniecka, P., Danku, J., Koprivova, A., Lahner, B., Luo, H., …Salt, D. E. (2014). Variation in sulfur and selenium accumulation is controlled by naturally occurring isoforms of the key sulfur assimilation enzyme ADENOSINE 5′-PHOSPHOSULFATE REDUCTASE2 across the arabidopsis species range. Plant Physiology, 166(3), https://doi.org/10.1104/pp.114.247825
DOI https://doi.org/10.1104/pp.114.247825
Publisher URL http://www.plantphysiol.org/content/166/3/1593
Copyright Statement Copyright information regarding this work can be found at the following address: http://eprints.nottingh.../end_user_agreement.pdf

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Copyright Statement
Copyright information regarding this work can be found at the following address: http://eprints.nottingham.ac.uk/end_user_agreement.pdf





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