Rational drug repurposing using sscMap analysis in a HOX-TALE model of leukemia

Kettyle, Laura M.J.; Liberante, Fabio G.; Thompson, Alexander

Abstract

Drug discovery and development are often hampered by lack of target identification and clinical tractability. Repurposing of approved drugs to life-threatening diseases such as leukemia is emerging as a promising alternative approach. Connectivity mapping systems link approved drugs with disease-related gene signatures. Relevant preclinical models provide essential tools for system validation and proof-of-concept studies. Herein we describe procedures aimed at generating disease-based gene signatures and applying them to established cross-referencing databases of potential candidate drugs. As a proof of principle, we present the identification of Entinostat as a candidate drug for the treatment of HOX TALE-related leukemia.

Citation

Kettyle, L. M., Liberante, F. G., & Thompson, A. (2014). Rational drug repurposing using sscMap analysis in a HOX-TALE model of leukemia. Methods in Molecular Biology, 1196, https://doi.org/10.1007/978-1-4939-1242-1_21

• Journal Article Type: Article
• Acceptance Date: May 8, 2014
• Publication Date: Jul 8, 2014
• Deposit Date: May 12, 2017
• Journal: Methods in Molecular Biology
• Electronic ISSN: 1940-6029
• Peer Reviewed: Peer Reviewed
• Volume: 1196
• DOI: https://doi.org/10.1007/978-1-4939-1242-1_21
• Keywords: HOXA9 Meis1 Data mining Gene expression profiles Connectivity mapping (sscMap) Leukemia Entinostat Preclinical model
• Public URL: https://nottingham-repository.worktribe.com/output/732830
• Publisher URL: https://link.springer.com/protocol/10.1007%2F978-1-4939-1242-1_21
• Additional Information: The final publication is available at Springer via http://dx.doi.org/10.1007/978-1-4939-1242-1_21