Y. Sun
Naturally Occurring Mutations in the PA Gene Are Key Contributors to Increased Virulence of Pandemic H1N1/09 Influenza Virus in Mice
Sun, Y.; Xu, Q.; Shen, Y.; Liu, L.; Wei, K.; Sun, H.; Pu, J.; Chang, K.-C.; Liu, J.
Authors
Q. Xu
Y. Shen
L. Liu
K. Wei
H. Sun
J. Pu
Professor KIN-CHOW CHANG KIN-CHOW.CHANG@NOTTINGHAM.AC.UK
PROFESSOR OF VETERINARY MOLECULAR MEDICINE
J. Liu
Abstract
We examined the molecular basis of virulence of pandemic H1N1/09 influenza viruses by reverse genetics based on two H1N1/09 virus isolates (A/California/04/2009 [CA04] and A/swine/Shandong/731/2009 [SD731]) with contrasting pathogenicities in mice. We found that four amino acid mutations (P224S in the PA protein [PA-P224S], PB2-T588I, NA-V106I, and NS1-I123V) contributed to the lethal phenotype of SD731. In particular, the PA-P224S mutation when combined with PA-A70V in CA04 drastically reduced the virus's 50% mouse lethal dose (LD50), by almost 1,000-fold.
Citation
Sun, Y., Xu, Q., Shen, Y., Liu, L., Wei, K., Sun, H., Pu, J., Chang, K.-C., & Liu, J. (2014). Naturally Occurring Mutations in the PA Gene Are Key Contributors to Increased Virulence of Pandemic H1N1/09 Influenza Virus in Mice. Journal of Virology, 88(8), 4600-4604. https://doi.org/10.1128/jvi.03158-13
Journal Article Type | Article |
---|---|
Acceptance Date | Feb 3, 2014 |
Online Publication Date | Mar 26, 2014 |
Publication Date | Apr 15, 2014 |
Deposit Date | Jun 30, 2017 |
Journal | Journal of Virology |
Print ISSN | 0022-538X |
Electronic ISSN | 1098-5514 |
Publisher | American Society for Microbiology |
Peer Reviewed | Peer Reviewed |
Volume | 88 |
Issue | 8 |
Pages | 4600-4604 |
DOI | https://doi.org/10.1128/jvi.03158-13 |
Public URL | https://nottingham-repository.worktribe.com/output/731501 |
Publisher URL | http://jvi.asm.org/content/88/8/4600 |
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