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Campylobacter jejuni gene cj0511 encodes a serine peptidase essential for colonisation

Karlyshev, A.V.; Thacker, G.; Jones, M.A.; Clements, M.O.; Wren, Brendan W.

Authors

A.V. Karlyshev

G. Thacker

M.O. Clements

Brendan W. Wren brendan.wren@lshtm.ac.uk



Abstract

According to MEROPS peptidase database, Campylobacter species encode 64 predicted peptidases. However, proteolytic properties of only a few of these proteins have been confirmed experimentally. In this study we identified and characterised a Campylobacter jejuni gene cj0511 encoding a novel peptidase. The proteolytic activity associated with this enzyme was demonstrated in cell lysates. Moreover, enzymatic studies conducted with a purified protein confirmed a prediction of it being a serine peptidase. Furthermore, cj0511 mutant was found to be severely attenuated in chicken colonisation model, suggesting a role of the Cj0511 protein in infection.

Journal Article Type Article
Publication Date May 9, 2014
Journal FEBS Open Bio
Electronic ISSN 2211-5463
Publisher Wiley
Peer Reviewed Peer Reviewed
Volume 4
Issue 1
APA6 Citation Karlyshev, A., Thacker, G., Jones, M., Clements, M., & Wren, B. W. (2014). Campylobacter jejuni gene cj0511 encodes a serine peptidase essential for colonisation. FEBS Open Bio, 4(1), https://doi.org/10.1016/j.fob.2014.04.012
DOI https://doi.org/10.1016/j.fob.2014.04.012
Keywords Campylobacter, Protease, Colonisation, Chicken
Publisher URL http://onlinelibrary.wiley.com/doi/10.1016/j.fob.2014.04.012/epdf
Copyright Statement Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by-nc-nd/4.0

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Copyright Statement
Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by-nc-nd/4.0





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