Campylobacter jejuni gene cj0511 encodes a serine peptidase essential for colonisation
Karlyshev, A.V.; Thacker, G.; Jones, M.A.; Clements, M.O.; Wren, Brendan W.
MICHAEL JONES email@example.com
Brendan W. Wren firstname.lastname@example.org
According to MEROPS peptidase database, Campylobacter species encode 64 predicted peptidases. However, proteolytic properties of only a few of these proteins have been confirmed experimentally. In this study we identified and characterised a Campylobacter jejuni gene cj0511 encoding a novel peptidase. The proteolytic activity associated with this enzyme was demonstrated in cell lysates. Moreover, enzymatic studies conducted with a purified protein confirmed a prediction of it being a serine peptidase. Furthermore, cj0511 mutant was found to be severely attenuated in chicken colonisation model, suggesting a role of the Cj0511 protein in infection.
|Journal Article Type||Article|
|Publication Date||May 9, 2014|
|Journal||FEBS Open Bio|
|Peer Reviewed||Peer Reviewed|
|APA6 Citation||Karlyshev, A., Thacker, G., Jones, M., Clements, M., & Wren, B. W. (2014). Campylobacter jejuni gene cj0511 encodes a serine peptidase essential for colonisation. FEBS Open Bio, 4(1), https://doi.org/10.1016/j.fob.2014.04.012|
|Keywords||Campylobacter, Protease, Colonisation, Chicken|
|Copyright Statement||Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by-nc-nd/4.0|
Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by-nc-nd/4.0
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