Victor V. Volkov
Mapping blood biochemistry by Raman spectroscopy at the cellular level
Volkov, Victor V.; McMaster, Jonathan; Aizenberg, Joanna; Perry, Carole C.
Authors
JONATHAN MCMASTER Jonathan.McMaster@nottingham.ac.uk
Professor of Chemistry
Joanna Aizenberg
Carole C. Perry
Abstract
We report how Raman difference imaging provides insight on cellular biochemistryin vivoas a function of sub-cellular dimensions and the cellular environment. We show that this approach offers a sensitive diagnostic to address blood biochemistry at the cellular level. We examine Raman microscopic images of the distribution of the different hemoglobins in both healthy (discocyte) and unhealthy (echinocyte) blood cells and interpret these images using pre-calculated, accurate pre-resonant Raman tensors for scattering intensities specific to hemoglobins. These tensors are developed from theoretical calculations of models of the oxy, deoxy and met forms of heme benchmarked against the experimental visible spectra of the corresponding hemoglobins. The calculations also enable assignments of the electronic transitions responsible for the colour of blood: these are mainly ligand to metal charge transfer transitions.
Citation
Volkov, V. V., McMaster, J., Aizenberg, J., & Perry, C. C. (2022). Mapping blood biochemistry by Raman spectroscopy at the cellular level. Chemical Science, 13(1), 133-140. https://doi.org/10.1039/d1sc05764b
Journal Article Type | Article |
---|---|
Acceptance Date | Dec 1, 2021 |
Online Publication Date | Dec 1, 2021 |
Publication Date | Jan 7, 2022 |
Deposit Date | Jan 7, 2022 |
Publicly Available Date | Jan 12, 2022 |
Journal | Chemical Science |
Print ISSN | 2041-6520 |
Electronic ISSN | 2041-6539 |
Publisher | Royal Society of Chemistry |
Peer Reviewed | Peer Reviewed |
Volume | 13 |
Issue | 1 |
Pages | 133-140 |
DOI | https://doi.org/10.1039/d1sc05764b |
Keywords | General Chemistry |
Public URL | https://nottingham-repository.worktribe.com/output/7171366 |
Publisher URL | https://pubs.rsc.org/en/content/articlelanding/2022/SC/D1SC05764B |
Files
D1sc05764b
(670 Kb)
PDF
Publisher Licence URL
https://creativecommons.org/licenses/by/3.0/
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