Signature-tagged transposon mutagenesis identifies novel mycobacterium tuberculosis genes involved in the parasitism of human macrophages

Rosas-Magallanes, Vania; Stadthagen-Gomez, Gustavo; Rauzier, Jean; Barreiro, Luis B.; Tailleux, Ludovic; Boudou, Frédéric; Griffin, Ruth; Nigou, Jérome; Jackson, Mary; Gicquel, Brigittte; Neyrolles, Olivier

Abstract

Using signature-tagged transposon mutagenesis, we isolated 23 Mycobacterium tuberculosis mutants, corresponding to 21 genes or genetic regions, attenuated in their ability to parasitize human macrophages. Mutants disrupted in the ABC transporter-encoding genes Rv0986 and Rv0987 were further characterized as being impaired in their ability to bind to host cells.

Citation

Rosas-Magallanes, V., Stadthagen-Gomez, G., Rauzier, J., Barreiro, L. B., Tailleux, L., Boudou, F., …Neyrolles, O. (2007). Signature-tagged transposon mutagenesis identifies novel mycobacterium tuberculosis genes involved in the parasitism of human macrophages. Infection and Immunity, 75(1), doi:10.1128/IAI.00058-06

• Journal Article Type: Article
• Acceptance Date: Sep 26, 2006
• Online Publication Date: Oct 9, 2006
• Publication Date: Jan 1, 2007
• Deposit Date: Dec 13, 2017
• Journal: Infection and Immunity
• Print ISSN: 0019-9567
• Electronic ISSN: 1098-5522
• Publisher: American Society for Microbiology
• Peer Reviewed: Peer Reviewed
• Volume: 75
• Issue: 1
• DOI: https://doi.org/10.1128/IAI.00058-06.
• Public URL: http://eprints.nottingham.ac.uk/id/eprint/48696
• Publisher URL: http://iai.asm.org/content/75/1/504
• Copyright Statement: Copyright information regarding this work can be found at the following address: http://eprints.nottingh.../end_user_agreement.pdf