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Poroelastic osmoregulation of living cell volume

Esteki, Mohammad Hadi; Malandrino, Andrea; Alemrajabi, Ali Akbar; Sheridan, Graham K.; Charras, Guillaume; Moeendarbary, Emad

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Authors

Mohammad Hadi Esteki

Andrea Malandrino

Ali Akbar Alemrajabi

Guillaume Charras

Emad Moeendarbary



Abstract

Cells maintain their volume through fine intracellular osmolarity regulation. Osmotic challenges drive fluid into or out of cells causing swelling or shrinkage, respectively. The dynamics of cell volume changes depending on the rheology of the cellular constituents and on how fast the fluid permeates through the membrane and cytoplasm. We investigated whether and how poroelasticity can describe volume dynamics in response to osmotic shocks. We exposed cells to osmotic perturbations and used defocusing epifluorescence microscopy on membrane-attached fluorescent nanospheres to track volume dynamics with high spatiotemporal resolution. We found that a poroelastic model that considers both geometrical and pressurization rates captures fluid-cytoskeleton interactions, which are rate-limiting factors in controlling volume changes at short timescales. Linking cellular responses to osmotic shocks and cell mechanics through poroelasticity can predict the cell state in health, disease, or in response to novel therapeutics.

Citation

Esteki, M. H., Malandrino, A., Alemrajabi, A. A., Sheridan, G. K., Charras, G., & Moeendarbary, E. (2021). Poroelastic osmoregulation of living cell volume. iScience, 24(12), Article 103482. https://doi.org/10.1016/j.isci.2021.103482

Journal Article Type Article
Acceptance Date Nov 19, 2021
Online Publication Date Nov 22, 2021
Publication Date Dec 17, 2021
Deposit Date Dec 10, 2021
Publicly Available Date Dec 13, 2021
Journal iScience
Electronic ISSN 2589-0042
Publisher Cell Press
Peer Reviewed Peer Reviewed
Volume 24
Issue 12
Article Number 103482
DOI https://doi.org/10.1016/j.isci.2021.103482
Keywords Multidisciplinary
Public URL https://nottingham-repository.worktribe.com/output/6916566
Publisher URL https://www.sciencedirect.com/science/article/pii/S258900422101453X?via%3Dihub

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