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Identification of the Genome Segments of Bluetongue Virus Type 26/Type 1 Reassortants Influencing Horizontal Transmission in a Mouse Model

Attoui, Houssam; Monsion, Baptiste; Klonjkowski, Bernard; Zientara, Stéphan; Mertens, Peter P. C.; Mohd Jaafar, Fauziah

Identification of the Genome Segments of Bluetongue Virus Type 26/Type 1 Reassortants Influencing Horizontal Transmission in a Mouse Model Thumbnail


Authors

Houssam Attoui

Baptiste Monsion

Bernard Klonjkowski

Stéphan Zientara

Fauziah Mohd Jaafar



Abstract

Bluetongue virus serotypes 1 to 24 are transmitted primarily by infected Culicoides midges, in which they also replicate. However, “atypical” BTV serotypes (BTV‐25, ‐26, ‐27 and ‐28) have recently been identified that do not infect and replicate in adult Culicoides, or a Culicoides derived cell line (KC cells). These atypical viruses are transmitted horizontally by direct contact between infected and susceptible hosts (primarily small ruminants) causing only mild clinical signs, although the exact transmission mechanisms involved have yet to be determined. We used reverse genetics to generate a strain of BTV‐1 (BTV‐1 RGC7) which is less virulent, infecting IFNAR(−/−) mice without killing them. Reassortant viruses were also engineered, using the BTV‐1 RGC7 genetic backbone, containing individual genome segments derived from BTV‐26. These reassortant viruses were used to explore the genetic control of horizontal transmission (HT) in the IFNAR(−/−) mouse model. Previous studies showed that genome segments 1, 2 and 3 restrict infection of Culicoides cells, along with a minor role for segment 7. The current study demonstrates that genome segments 2, 5 and 10 of BTV‐26 (coding for proteins VP2, NS1 and NS3/NS3a/NS5, respectively) are individually sufficient to promote HT.

Citation

Attoui, H., Monsion, B., Klonjkowski, B., Zientara, S., Mertens, P. P. C., & Mohd Jaafar, F. (2021). Identification of the Genome Segments of Bluetongue Virus Type 26/Type 1 Reassortants Influencing Horizontal Transmission in a Mouse Model. Viruses, 13(11), Article 2208. https://doi.org/10.3390/v13112208

Journal Article Type Article
Acceptance Date Oct 28, 2021
Online Publication Date Nov 2, 2021
Publication Date Nov 1, 2021
Deposit Date Nov 5, 2021
Publicly Available Date Nov 5, 2021
Journal Viruses
Electronic ISSN 1999-4915
Publisher MDPI AG
Peer Reviewed Peer Reviewed
Volume 13
Issue 11
Article Number 2208
DOI https://doi.org/10.3390/v13112208
Keywords bluetongue; bluetongue virus; BTV; BTV‐1; BTV‐26; horizontal transmission; vector independent transmission; reassortment; IFNAR(−/−) mice
Public URL https://nottingham-repository.worktribe.com/output/6613334
Publisher URL https://www.mdpi.com/1999-4915/13/11/2208

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