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Identification of Orbivirus Non-Structural Protein 5 (NS5), Its Role and Interaction with RNA/DNA in Infected Cells

Mohd Jaafar, Fauziah; Monsion, Baptiste; Mertens, Peter P.C.; Attoui, Houssam

Identification of Orbivirus Non-Structural Protein 5 (NS5), Its Role and Interaction with RNA/DNA in Infected Cells Thumbnail


Authors

Fauziah Mohd Jaafar

Baptiste Monsion

Houssam Attoui



Abstract

Bioinformatic analyses have predicted that orbiviruses encode an additional, small non-structural protein (NS5) from a secondary open reading frame on genome segment 10. However, this protein has not previously been detected in infected mammalian or insect cells. NS5-specific antibodies were generated in mice and were used to identify NS5 synthesised in orbivirus-infected BSR cells or cells transfected with NS5 expression plasmids. Confocal microscopy shows that although NS5 accumulates in the nucleus, particularly in the nucleolus, which becomes disrupted, it also appears in the cell cytoplasm, co-localising with mitochondria. NS5 helps to prevent the degradation of ribosomal RNAs during infection and reduces host-cell protein synthesis However, it helps to extend cell viability by supporting viral protein synthesis and virus replication. Pulldown studies showed that NS5 binds to ssRNAs and supercoiled DNAs and demonstrates interactions with ZBP1, suggesting that it modulates host-cell responses.

Citation

Mohd Jaafar, F., Monsion, B., Mertens, P. P., & Attoui, H. (2023). Identification of Orbivirus Non-Structural Protein 5 (NS5), Its Role and Interaction with RNA/DNA in Infected Cells. International Journal of Molecular Sciences, 24(7), Article 6845. https://doi.org/10.3390/ijms24076845

Journal Article Type Article
Acceptance Date Mar 31, 2023
Online Publication Date Apr 6, 2023
Publication Date Apr 6, 2023
Deposit Date Apr 18, 2023
Publicly Available Date Apr 18, 2023
Journal International Journal of Molecular Sciences
Print ISSN 1661-6596
Electronic ISSN 1422-0067
Publisher MDPI AG
Peer Reviewed Peer Reviewed
Volume 24
Issue 7
Article Number 6845
DOI https://doi.org/10.3390/ijms24076845
Keywords bluetongue; bluetongue virus; orbivirus; African horsesickness virus; Kemerovo virus; AHSV; BTV; KEMV; NS5
Public URL https://nottingham-repository.worktribe.com/output/19298192
Publisher URL https://www.mdpi.com/1422-0067/24/7/6845

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