Impedimetric Characterization of Bipolar Nanoelectrodes with Cancer Cells

Robinson, Andie J.; Jain, Akhil; Rahman, Ruman; Abayzeed, Sidahmed; Hague, Richard J.M.; Rawson, Frankie J.

doi:10.1021/acsomega.1c03547

Impedimetric Characterization of Bipolar Nanoelectrodes with Cancer Cells

Robinson, Andie J.; Jain, Akhil; Rahman, Ruman; Abayzeed, Sidahmed; Hague, Richard J.M.; Rawson, Frankie J.

Authors

Andie J. Robinson

Akhil Jain

Professor Ruman Rahman RUMAN.RAHMAN@NOTTINGHAM.AC.UK
PROFESSOR OF MOLECULAR NEURO-ONCOLOGY

Dr SIDAHMED ABAYZEED SIDAHMED.ABAYZEED2@NOTTINGHAM.AC.UK
ASSISTANT PROFESSOR

Professor RICHARD HAGUE RICHARD.HAGUE@NOTTINGHAM.AC.UK
Professor of Additive Manufacturing

Dr Frankie Rawson Frankie.Rawson@nottingham.ac.uk
ASSOCIATE PROFESSOR

Abstract

Merging of electronics with biology, defined as bioelectronics, at the nanoscale holds considerable promise for sensing and modulating cellular behavior. Advancing our understanding of nanobioelectronics will facilitate development and enable applications in biosensing, tissue engineering, and bioelectronic medicine. However, studies investigating the electrical effects when merging wireless conductive nanoelectrodes with biology are lacking. Consequently, a tool is required to develop a greater understanding of merging conductive nanoparticles with cells. Herein, this challenge is addressed by developing an impedimetric method to evaluate bipolar electrode (BPE) systems that could report on electrical input. A theoretical framework is provided, using impedance to determine if conductive nanoparticles can be polarized and used to drive current. It is then demonstrated that 125 nm of gold nanoparticle (AuNP) bipolar electrodes (BPEs) could be sensed in the presence of cells when incorporated intracellularly at 500 μg/mL using water and phosphate-buffered saline (PBS) as electrolytes. These results highlight how nanoscale BPEs act within biological systems. This research will impact the rational design of using BPE systems in cells for both sensing and actuating applications.

Citation

Robinson, A. J., Jain, A., Rahman, R., Abayzeed, S., Hague, R. J., & Rawson, F. J. (2021). Impedimetric Characterization of Bipolar Nanoelectrodes with Cancer Cells. ACS Omega, 6(44), 29495-29505. https://doi.org/10.1021/acsomega.1c03547

Journal Article Type	Article
Acceptance Date	Sep 17, 2021
Online Publication Date	Oct 29, 2021
Publication Date	Nov 9, 2021
Deposit Date	Sep 23, 2021
Publicly Available Date	Oct 29, 2021
Journal	ACS Omega
Electronic ISSN	2470-1343
Publisher	American Chemical Society
Peer Reviewed	Peer Reviewed
Volume	6
Issue	44
Pages	29495-29505
DOI	https://doi.org/10.1021/acsomega.1c03547
Public URL	https://nottingham-repository.worktribe.com/output/6296586
Publisher URL	https://pubs.acs.org/doi/10.1021/acsomega.1c03547