Huda Alfardus
Intratumour heterogeneity in microRNAs expression regulates glioblastoma metabolism
Alfardus, Huda; de los Angeles Estevez Cebrero, Maria; Rowlinson, Jonathan; Lourdusamy, Anbarasu; Abdelrazig, Salah; Ortori, Catherine; Grundy, Richard; Kim, Dong-Hyun; McIntyre, Alan; Smith, Stuart
Authors
Maria de los Angeles Estevez Cebrero
Jonathan Rowlinson
AROCKIA LOURDUSAMY ANBARASU.LOURDUSAMY@NOTTINGHAM.AC.UK
Senior Research Fellow
Salah Abdelrazig
Catherine Ortori
RICHARD GRUNDY richard.grundy@nottingham.ac.uk
Professor of Paediatric Neuro-Oncology
DONG-HYUN KIM Dong-hyun.Kim@nottingham.ac.uk
Associate Professor
ALAN MCINTYRE ALAN.MCINTYRE@NOTTINGHAM.AC.UK
Professor of Molecular Oncology
STUART SMITH stuart.smith@nottingham.ac.uk
Clinical Associate Professor
Abstract
While specific microRNA (miRNA) signatures have been identified in glioblastoma (GBM), the intratumour heterogeneity in miRNA expression has not yet been characterised. In this study, we reveal significant alterations in miRNA expression across three GBM tumour regions: the core, rim, and invasive margin. Our miRNA profiling analysis showed that miR-330-5p and miR-215-5p were upregulated in the invasive margin relative to the core and the rim regions, while miR-619-5p, miR-4440 and miR-4793-3p were downregulated. Functional analysis of newly identified miRNAs suggests their involvement in regulating lipid metabolic pathways. Subsequent liquid chromatography–mass spectrometry (LC–MS) and tandem mass spectroscopy (LC–MS/MS) profiling of the intracellular metabolome and the lipidome of GBM cells with dysregulated miRNA expression confirmed the alteration in the metabolite levels associated with lipid metabolism. The identification of regional miRNA expression signatures may underlie the metabolic heterogeneity within the GBM tumour and understanding this relationship may open new avenues for the GBM treatment.
Citation
Alfardus, H., de los Angeles Estevez Cebrero, M., Rowlinson, J., Lourdusamy, A., Abdelrazig, S., Ortori, C., …Smith, S. (2021). Intratumour heterogeneity in microRNAs expression regulates glioblastoma metabolism. Scientific Reports, 11(1), Article 15908. https://doi.org/10.1038/s41598-021-95289-9
Journal Article Type | Article |
---|---|
Acceptance Date | Jul 16, 2021 |
Online Publication Date | Aug 5, 2021 |
Publication Date | Dec 1, 2021 |
Deposit Date | Jul 19, 2021 |
Publicly Available Date | Aug 9, 2021 |
Journal | Scientific Reports |
Electronic ISSN | 2045-2322 |
Publisher | Nature Publishing Group |
Peer Reviewed | Peer Reviewed |
Volume | 11 |
Issue | 1 |
Article Number | 15908 |
DOI | https://doi.org/10.1038/s41598-021-95289-9 |
Public URL | https://nottingham-repository.worktribe.com/output/5806377 |
Publisher URL | https://www.nature.com/articles/s41598-021-95289-9 |
Files
s41598-021-95289-9
(2.6 Mb)
PDF
Publisher Licence URL
https://creativecommons.org/licenses/by/4.0/
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