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Osteogenic Response of Human Mesenchymal Stem Cells Analysed Using Combined Intracellular and Extracellular Metabolomic Monitoring

Surrati, Amal; Evseev, Sergey; Jourdan, Fabien; Kim, Dong-Hyun; Sottile, Virginie

Osteogenic Response of Human Mesenchymal Stem Cells Analysed Using Combined Intracellular and Extracellular Metabolomic Monitoring Thumbnail


Authors

Amal Surrati

Sergey Evseev

Fabien Jourdan

Virginie Sottile



Abstract

Background/Aims: The skeleton is a metabolically active organ undergoing continuous remodelling initiated by mesenchymal progenitors present in bone and bone marrow. Under certain pathological conditions this remodelling balance shifts towards increased resorption resulting in weaker bone microarchitecture, and there is consequently a therapeutic need to identify pathways that could inversely enhance bone formation from stem cells. Metabolomics approaches recently applied to stem cell characterisation could help identify new biochemical markers involved in osteogenic differentiation. Methods: Combined intra- and extracellular metabolite profiling was performed by liquid chromatography-mass spectrometry (LC-MS) on human mesenchymal stem cells (MSCs) undergoing osteogenic differentiation in vitro. Using a combination of univariate and multivariate analyses, changes in metabolite and nutrient concentration were monitored in cultures under osteogenic treatment over 10 days. Results: A subset of differentially detected compounds was identified in differentiating cells, suggesting a direct link to metabolic processes involved in osteogenic response. Conclusion: These results highlight new metabolite candidates as potential biomarkers to monitor stem cell differentiation towards the bone lineage.

Citation

Surrati, A., Evseev, S., Jourdan, F., Kim, D.-H., & Sottile, V. (2021). Osteogenic Response of Human Mesenchymal Stem Cells Analysed Using Combined Intracellular and Extracellular Metabolomic Monitoring. Cellular Physiology and Biochemistry, 55(3), 311-326. https://doi.org/10.33594/000000377

Journal Article Type Article
Acceptance Date May 6, 2021
Online Publication Date Jun 19, 2021
Publication Date Jun 19, 2021
Deposit Date May 25, 2021
Publicly Available Date Jun 19, 2021
Journal Cellular Physiology and Biochemistry
Print ISSN 1015-8987
Electronic ISSN 1421-9778
Publisher Karger Publishers
Peer Reviewed Peer Reviewed
Volume 55
Issue 3
Pages 311-326
DOI https://doi.org/10.33594/000000377
Keywords Mesenchymal stem cells; osteogenesis; differentiation; metabolomics; LC-MS; metabolomics profiling
Public URL https://nottingham-repository.worktribe.com/output/5571712
Publisher URL https://www.cellphysiolbiochem.com/Articles/000377/

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