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Sequential Orbitrap Secondary Ion Mass Spectrometry and Liquid Extraction Surface Analysis-Tandem Mass Spectrometry-Based Metabolomics for Prediction of Brain Tumor Relapse from Sample-Limited Primary Tissue Archives

Meurs, Joris; Scurr, David J.; Lourdusamy, Anbarasu; Storer, Lisa C.D.; Grundy, Richard G.; Alexander, Morgan R.; Rahman, Ruman; Kim, Dong-Hyun

Sequential Orbitrap Secondary Ion Mass Spectrometry and Liquid Extraction Surface Analysis-Tandem Mass Spectrometry-Based Metabolomics for Prediction of Brain Tumor Relapse from Sample-Limited Primary Tissue Archives Thumbnail


Authors

Joris Meurs

DAVID SCURR DAVID.SCURR@NOTTINGHAM.AC.UK
Principal Research Fellow

Lisa C.D. Storer

RICHARD GRUNDY richard.grundy@nottingham.ac.uk
Professor of Paediatric Neuro-Oncology

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MORGAN ALEXANDER MORGAN.ALEXANDER@NOTTINGHAM.AC.UK
Professor of Biomedical Surfaces



Abstract

We present here a novel surface mass spectrometry strategy to perform untargeted metabolite profiling of formalin-fixed paraffin-embedded pediatric ependymoma archives. Sequential Orbitrap secondary ion mass spectrometry (3D OrbiSIMS) and liquid extraction surface analysis-tandem mass spectrometry (LESA-MS/MS) permitted the detection of 887 metabolites (163 chemical classes) from pediatric ependymoma tumor tissue microarrays (diameter: <1 mm; thickness: 4 ?m). From these 163 classes, 60 classes were detected with both techniques, whilst LESA-MS/MS and 3D OrbiSIMS individually allowed the detection of another 83 and 20 unique metabolite classes, respectively. Through data fusion and multivariate analysis, we were able to identify key metabolites and corresponding pathways predictive of tumor relapse, which were retrospectively confirmed by gene expression analysis with publicly available data. Altogether, this sequential mass spectrometry strategy has shown to be a versatile tool to perform high-throughput metabolite profiling on sample-limited tissue archives.

Journal Article Type Article
Acceptance Date Mar 25, 2021
Online Publication Date Apr 26, 2021
Publication Date May 11, 2021
Deposit Date Apr 16, 2021
Publicly Available Date Apr 27, 2022
Journal Analytical Chemistry
Print ISSN 0003-2700
Electronic ISSN 1520-6882
Publisher American Chemical Society
Peer Reviewed Peer Reviewed
Volume 93
Issue 18
Pages 6947-6954
DOI https://doi.org/10.1021/acs.analchem.0c05087
Keywords Analytical Chemistry
Public URL https://nottingham-repository.worktribe.com/output/5468987
Publisher URL https://pubs.acs.org/doi/10.1021/acs.analchem.0c05087

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