Leigh A. Stoddart
Efficient G protein coupling is not required for agonist‐mediated internalization and membrane reorganization of the adenosine A 3 receptor
Stoddart, Leigh A.; Kilpatrick, Laura E.; Corriden, Ross; Kellam, Barrie; Briddon, Stephen J.; Hill, Stephen J.
Authors
Doctor LAURA KILPATRICK LAURA.KILPATRICK@NOTTINGHAM.AC.UK
Assistant Professor
Ross Corriden
BARRIE KELLAM BARRIE.KELLAM@NOTTINGHAM.AC.UK
Professor of Medicinal Chemistry
STEPHEN BRIDDON stephen.briddon@nottingham.ac.uk
Principal Research Fellow
STEPHEN HILL STEVE.HILL@NOTTINGHAM.AC.UK
Professor of Molecular Pharmacology
Abstract
Organization of G protein-coupled receptors at the plasma membrane has been the focus of much recent attention. Advanced microscopy techniques have shown that these receptors can be localized to discrete microdomains and reorganization upon ligand activation is crucial in orchestrating their signaling. Here, we have compared the membrane organization and downstream signaling of a mutant (R108A, R3.50A) of the adenosine A3 receptor (A3AR) to that of the wild-type receptor. Fluorescence Correlation Spectroscopy (FCS) studies with a fluorescent agonist (ABEA-X-BY630) demonstrated that both wild-type and mutant receptors bind agonist with high affinity but in subsequent downstream signaling assays the R108A mutation abolished agonist-mediated inhibition of cAMP production and ERK phosphorylation. In further FCS studies, both A3AR and A3AR R108A underwent similar agonist-induced increases in receptor density and molecular brightness which were accompanied by a decrease in membrane diffusion after agonist treatment. Using bimolecular fluorescence complementation, experiments showed that the R108A mutant retained the ability to recruit β-arrestin and these receptor/arrestin complexes displayed similar membrane diffusion and organization to that observed with wild-type receptors. These data demonstrate that effective G protein signaling is not a prerequisite for agonist-stimulated β-arrestin recruitment and membrane reorganization of the A3AR.
Citation
Stoddart, L. A., Kilpatrick, L. E., Corriden, R., Kellam, B., Briddon, S. J., & Hill, S. J. (2021). Efficient G protein coupling is not required for agonist‐mediated internalization and membrane reorganization of the adenosine A 3 receptor. FASEB Journal, 35(4), Article e21211. https://doi.org/10.1096/fj.202001729rr
Journal Article Type | Article |
---|---|
Acceptance Date | Nov 9, 2020 |
Online Publication Date | Mar 12, 2021 |
Publication Date | Apr 1, 2021 |
Deposit Date | Nov 26, 2020 |
Publicly Available Date | Mar 13, 2021 |
Journal | FASEB Journal |
Print ISSN | 0892-6638 |
Electronic ISSN | 1530-6860 |
Publisher | Federation of American Society of Experimental Biology (FASEB) |
Peer Reviewed | Peer Reviewed |
Volume | 35 |
Issue | 4 |
Article Number | e21211 |
DOI | https://doi.org/10.1096/fj.202001729rr |
Keywords | Genetics; Molecular Biology; Biochemistry; Biotechnology |
Public URL | https://nottingham-repository.worktribe.com/output/5070702 |
Publisher URL | https://faseb.onlinelibrary.wiley.com/doi/full/10.1096/fj.202001729RR |
Files
Efficient G protein coupling is not required for agonist‐mediated internalization and membrane reorganization of the adenosine A3 receptor
(1.1 Mb)
PDF
Publisher Licence URL
https://creativecommons.org/licenses/by/4.0/
You might also like
Fluorescently Labeled Morphine Derivatives for Bioimaging Studies
(2018)
Journal Article
Downloadable Citations
About Repository@Nottingham
Administrator e-mail: discovery-access-systems@nottingham.ac.uk
This application uses the following open-source libraries:
SheetJS Community Edition
Apache License Version 2.0 (http://www.apache.org/licenses/)
PDF.js
Apache License Version 2.0 (http://www.apache.org/licenses/)
Font Awesome
SIL OFL 1.1 (http://scripts.sil.org/OFL)
MIT License (http://opensource.org/licenses/mit-license.html)
CC BY 3.0 ( http://creativecommons.org/licenses/by/3.0/)
Powered by Worktribe © 2024
Advanced Search