Uclacyanin Proteins Are Required for Lignified Nanodomain Formation within Casparian Strips

Reyt, Guilhem; Chao, Zhenfei; Flis, Paulina; Salas-Gonz�lez, Isai; Castrillo, Gabriel; Chao, Dai Yin; Salt, David E.

Abstract

© 2020 The Author(s) Casparian strips (CSs) are cell wall modifications of vascular plants restricting extracellular free diffusion into and out of the vascular system [1]. This barrier plays a critical role in controlling the acquisition of nutrients and water necessary for normal plant development [2–5]. CSs are formed by the precise deposition of a band of lignin approximately 2 μm wide and 150 nm thick spanning the apoplastic space between adjacent endodermal cells [6, 7]. Here, we identified a copper-containing protein, Uclacyanin1 (UCC1), that is sub-compartmentalized within the CS. UCC1 forms a central CS nanodomain in comparison with other CS-located proteins that are found to be mainly accumulated at the periphery of the CS. We found that loss-of-function of two uclacyanins (UCC1 and UCC2) reduces lignification specifically in this central CS nanodomain, revealing a nano-compartmentalized machinery for lignin polymerization. This loss of lignification leads to increased endodermal permeability and, consequently, to a loss of mineral nutrient homeostasis.

• Journal Article Type: Article
• Acceptance Date: Jul 28, 2020
• Online Publication Date: Aug 27, 2020
• Publication Date: Aug 27, 2020
• Deposit Date: Sep 14, 2020
• Publicly Available Date: Sep 14, 2020
• Journal: Current Biology
• Print ISSN: 0960-9822
• Electronic ISSN: 1879-0445
• Publisher: Cell Press
• Peer Reviewed: Peer Reviewed
• Volume: 30
• Issue: 20
• Pages: 4103-4111.e6
• DOI: https://doi.org/10.1016/j.cub.2020.07.095
• Keywords: General Biochemistry, Genetics and Molecular Biology; General Agricultural and Biological Sciences
• Public URL: https://nottingham-repository.worktribe.com/output/4869523
• Publisher URL: https://www.cell.com/current-biology/fulltext/S0960-9822(20)31154-4?_returnURL=https%3A%2F%2Flinkinghub.elsevier.com%2Fretrieve%2Fpii%2FS0960982220311544%3Fshowall%3Dtrue