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Pro-organic radical contrast agents (“pro-ORCAs”) for real-time MRI of pro-drug activation in biological systems

Nguyen, Hung V.-T.; Detappe, Alexandre; Harvey, Peter; Gallagher, Nolan; Mathieu, Clelia; Agius, Michael P.; Zavidij, Oksana; Wang, Wencong; Jiang, Yivan; Rajca, Andrzej; Jasanoff, Alan; Ghobrial, Irene M.; Ghoroghchian, P. Peter; Johnson, Jeremiah A.

Pro-organic radical contrast agents (“pro-ORCAs”) for real-time MRI of pro-drug activation in biological systems Thumbnail


Authors

Hung V.-T. Nguyen

Alexandre Detappe

Nolan Gallagher

Clelia Mathieu

Michael P. Agius

Oksana Zavidij

Wencong Wang

Yivan Jiang

Andrzej Rajca

Alan Jasanoff

Irene M. Ghobrial

P. Peter Ghoroghchian

Jeremiah A. Johnson



Abstract

Nitroxide-based organic-radical contrast agents (ORCAs) are promising as safe next-generation magnetic resonance imaging (MRI) tools. Nevertheless, stimuli-responsive ORCAs that enable MRI monitoring of prodrug activation have not been reported; such systems could open new avenues for prodrug validation and image-guided drug delivery. Here, we introduce a novel “pro-ORCA” concept that addresses this challenge. By covalent conjugation of nitroxides and drug molecules (doxorubicin, DOX) to the same brush-arm star polymer (BASP) through chemically identical cleavable linkers, we demonstrate that pro-ORCA and prodrug activation, i.e., ORCA and DOX release, leads to significant changes in MRI contrast that correlate with cytotoxicity. This approach is shown to be general for a range of commonly used linker cleavage mechanisms (e.g., photolysis and hydrolysis) and release rates. Pro-ORCAs could find applications as research tools or clinically viable “reporter theranostics” for in vitro and in vivo MRI-correlated prodrug activation.

Citation

Nguyen, H. V.-T., Detappe, A., Harvey, P., Gallagher, N., Mathieu, C., Agius, M. P., …Johnson, J. A. (2020). Pro-organic radical contrast agents (“pro-ORCAs”) for real-time MRI of pro-drug activation in biological systems. Polymer Chemistry, 11(29), 4768-4779. https://doi.org/10.1039/d0py00558d

Journal Article Type Article
Acceptance Date Jun 22, 2020
Online Publication Date Jun 26, 2020
Publication Date Aug 7, 2020
Deposit Date Aug 5, 2020
Publicly Available Date Aug 6, 2020
Journal Polymer Chemistry
Print ISSN 1759-9954
Electronic ISSN 1759-9962
Publisher Royal Society of Chemistry
Peer Reviewed Peer Reviewed
Volume 11
Issue 29
Pages 4768-4779
DOI https://doi.org/10.1039/d0py00558d
Keywords Organic Chemistry; Biochemistry; Bioengineering; Polymers and Plastics
Public URL https://nottingham-repository.worktribe.com/output/4759747
Publisher URL https://pubs.rsc.org/en/content/articlelanding/2020/py/d0py00558d#!divAbstract
Additional Information : This document is Similarity Check deposited; : Supplementary Information; : Hung V.-T. Nguyen (ORCID); : Alexandre Detappe (ORCID); : Peter Harvey (ORCID); : Peter Harvey (ResearcherID); : Andrzej Rajca (ORCID); : Jeremiah A. Johnson (ORCID); : Jeremiah A. Johnson (ResearcherID); : Single-blind; : Received 17 April 2020; Accepted 22 June 2020; Accepted Manuscript published 26 June 2020; Advance Article published 8 July 2020

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