The Amino Acid Transporter JhI-21 Coevolves with Glutamate Receptors, Impacts NMJ Physiology and Influences Locomotor Activity in Drosophila Larvae

Ziegler, Anna B.; Augustin, Hrvoje; Clark, Nathan L.; Berthelot-Grosjean, Martine; Simonnet, M�gane M.; Steinert, Joern R.; Geillon, Flore; Mani�re, G�rard; Featherstone, David E.; Grosjean, Yael

Abstract

Changes in synaptic physiology underlie neuronal network plasticity and behavioral phenomena, which are adjusted during development. The Drosophila larval glutamatergic neuromuscular junction (NMJ) represents a powerful synaptic model to investigate factors impacting these processes. Amino acids such as glutamate have been shown to regulate Drosophila NMJ physiology by modulating the clustering of postsynaptic glutamate receptors and thereby regulating the strength of signal transmission from the motor neuron to the muscle cell. To identify amino acid transporters impacting glutmatergic signal transmission, we used Evolutionary Rate Covariation (ERC), a recently developed bioinformatic tool. Our screen identified ten proteins co-evolving with NMJ glutamate receptors. We selected one candidate transporter, the SLC7 (Solute Carrier) transporter family member JhI-21 (Juvenile hormone Inducible-21), which is expressed in Drosophila larval motor neurons. We show that JhI-21 suppresses postsynaptic muscle glutamate receptor abundance and that JhI-21 expression in motor neurons regulates larval crawling behavior in a developmental stage-specific manner.

Citation

Ziegler, A. B., Augustin, H., Clark, N. L., Berthelot-Grosjean, M., Simonnet, M. M., Steinert, J. R., …Grosjean, Y. (2016). The Amino Acid Transporter JhI-21 Coevolves with Glutamate Receptors, Impacts NMJ Physiology and Influences Locomotor Activity in Drosophila Larvae. Scientific Reports, 6(1), Article 19692. https://doi.org/10.1038/srep19692

• Journal Article Type: Article
• Acceptance Date: Dec 16, 2015
• Online Publication Date: Jan 25, 2016
• Publication Date: 2016-04
• Deposit Date: Jun 17, 2020
• Publicly Available Date: Jun 22, 2020
• Journal: Scientific Reports
• Electronic ISSN: 2045-2322
• Publisher: Nature Publishing Group
• Peer Reviewed: Peer Reviewed
• Volume: 6
• Issue: 1
• Article Number: 19692
• DOI: https://doi.org/10.1038/srep19692
• Keywords: Multidisciplinary
• Public URL: https://nottingham-repository.worktribe.com/output/4668271
• Publisher URL: https://www.nature.com/articles/srep19692
• Additional Information: Received: 24 July 2015; Accepted: 16 December 2015; First Online: 25 January 2016; : The authors declare no competing financial interests.