Auxin-dependent post-translational regulation of MONOPTEROS in the Arabidopsis root

Cavalleri, Alex; Astori, Chiara; Truskina, Jekaterina; Cucinotta, Mara; Farcot, Etienne; Chrysanthou, Elina; Xu, Xiaocai; Muino, Jose M.; Kaufmann, Kerstin; Kater, Martin M.; Vernoux, Teva; Weijers, Dolf; Bennett, Malcolm J.; Bhosale, Rahul; Bishopp, Anthony; Colombo, Lucia

Abstract

Auxin plays a pivotal role in plant development by activating AUXIN RESPONSE FACTORs (ARFs). Under low auxin levels, ARF activity is inhibited by interacting with Aux/IAAs. Aux/IAAs are degraded when the cellular auxin concentration increases, causing the release of ARF inhibition. Here, we show that levels of the ARF5/MONOPTEROS (MP) protein are regulated in a cell-type-specific and isoform-dependent manner. We find that the stability of MP isoforms is differentially controlled depending on the auxin level. The canonical MP isoform is degraded by the proteasome in root tissues with low auxin levels. While auxin sharpens the MP localization domain in roots, it does not do so in ovules or embryos. Our research highlights a mechanism for providing spatial control of auxin signaling capacity. Together with recent advances in understanding the tissue-specific expression and post-transcriptional modification of auxin signaling components, these results provide insights into understanding how auxin can elicit so many distinct responses.

Citation

Cavalleri, A., Astori, C., Truskina, J., Cucinotta, M., Farcot, E., Chrysanthou, E., Xu, X., Muino, J. M., Kaufmann, K., Kater, M. M., Vernoux, T., Weijers, D., Bennett, M. J., Bhosale, R., Bishopp, A., & Colombo, L. (2024). Auxin-dependent post-translational regulation of MONOPTEROS in the Arabidopsis root. Cell Reports, 43(12), Article 115083. https://doi.org/10.1016/j.celrep.2024.115083

• Journal Article Type: Article
• Acceptance Date: Nov 26, 2024
• Online Publication Date: Dec 15, 2024
• Publication Date: Dec 24, 2024
• Deposit Date: Mar 24, 2025
• Publicly Available Date: Mar 24, 2025
• Journal: Cell Reports
• Print ISSN: 2211-1247
• Electronic ISSN: 2211-1247
• Publisher: Cell Press
• Peer Reviewed: Peer Reviewed
• Volume: 43
• Issue: 12
• Article Number: 115083
• DOI: https://doi.org/10.1016/j.celrep.2024.115083
• Public URL: https://nottingham-repository.worktribe.com/output/44824393
• Publisher URL: https://www.sciencedirect.com/science/article/pii/S2211124724014347?via%3Dihub