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The CEP5 peptide promotes abiotic stress tolerance, as revealed by quantitative proteomics, and attenuates the AUX/IAA equilibrium in Arabidopsis

Smith, Stephanie; Zhu, Shanshuo; Joos, Lisa; Roberts, Ianto; Nikonorova, Natalia; Vu, Lam Dai; Stes, Elisabeth; Cho, Hyunwoo; Larrieu, Antoine; Xuan, Wei; Goodall, Benjamin; Cotte, Brigitte van de; Waite, Jessic Marie; Rigal, Adeline; Harborough, Sigurd R.R.; Persiau, Geert; Vanneste, Steffen; Kirschner, Gwendolyn K.; Vandermarliere, Elien; Martens, Lennart; Stahl, Yvonne; Audenaert, Dominique; Friml, Jiri; Felix, Georg; Simon, Ruediger; Bennett, Malcolm; Bishopp, Anthony; De Jaeger, Geert; Ljung, Karin; Kepinski, Stefan; Robert, Stephanie; Nemhauser, Jennifer; Hwang, Ildoo; Gevaert, Kris; Beeckman, Tom; De Smet, Ive

Authors

Stephanie Smith

Shanshuo Zhu

Lisa Joos

Ianto Roberts

Natalia Nikonorova

Lam Dai Vu

Elisabeth Stes

Hyunwoo Cho

Antoine Larrieu

Wei Xuan

Benjamin Goodall

Brigitte van de Cotte

Jessic Marie Waite

Adeline Rigal

Sigurd R.R. Harborough

Geert Persiau

Steffen Vanneste

Gwendolyn K. Kirschner

Elien Vandermarliere

Lennart Martens

Yvonne Stahl

Dominique Audenaert

Jiri Friml

Georg Felix

Ruediger Simon

Geert De Jaeger

Karin Ljung

Stefan Kepinski

Stephanie Robert

Jennifer Nemhauser

Ildoo Hwang

Kris Gevaert

Tom Beeckman

Ive De Smet



Abstract

Peptides derived from non-functional precursors play important roles in various developmental processes, but also in (a)biotic stress signaling. Our (phospho)proteome-wide analyses of C-terminally encoded peptide 5 (CEP5)-mediated changes revealed an impact on abiotic stress-related processes. Drought has a dramatic impact on plant growth, development and reproduction, and the plant hormone auxin plays a role in drought responses. Our genetic, physiological, biochemical and pharmacological results demonstrated that CEP5-mediated signaling is relevant for osmotic and drought stress tolerance in Arabidopsis, and that CEP5 specifically counteracts auxin effects. Specifically, we found that CEP5 signaling stabilizes AUX/IAA transcriptional repressors, suggesting the existence of a novel peptide-dependent control mechanism that tunes auxin signaling. These observations align with the recently described role of AUX/IAAs in stress tolerance and provide a novel role for CEP5 in osmotic and drought stress tolerance.

Citation

Smith, S., Zhu, S., Joos, L., Roberts, I., Nikonorova, N., Vu, L. D., …De Smet, I. (2020). The CEP5 peptide promotes abiotic stress tolerance, as revealed by quantitative proteomics, and attenuates the AUX/IAA equilibrium in Arabidopsis. Molecular and Cellular Proteomics, 19(8), 1248-1262. https://doi.org/10.1074/mcp.ra119.001826

Journal Article Type Article
Acceptance Date May 13, 2020
Online Publication Date May 13, 2020
Publication Date Aug 1, 2020
Deposit Date May 18, 2020
Publicly Available Date May 19, 2020
Journal Molecular and Cellular Proteomics
Print ISSN 1535-9476
Electronic ISSN 1535-9484
Publisher American Society for Biochemistry and Molecular Biology
Peer Reviewed Peer Reviewed
Volume 19
Issue 8
Pages 1248-1262
DOI https://doi.org/10.1074/mcp.ra119.001826
Keywords Analytical Chemistry; Biochemistry; Molecular Biology
Public URL https://nottingham-repository.worktribe.com/output/4463167
Publisher URL https://www.mcponline.org/content/early/2020/05/13/mcp.RA119.001826
Additional Information This research was originally published in the Journal of Biological Chemistry. Stephanie Smith, Shanshuo Zhu, Lisa Joos, Ianto Roberts, Natalia Nikonorova, Lam Dai Vu, Elisabeth Stes, Hyunwoo Cho, Antoine Larrieu, Wei Xuan, Benjamin Goodall, Brigitte van de Cotte, Jessic Marie Waite, Adeline Rigal, Sigurd R. R. Harborough, Geert Persiau, Steffen Vanneste, Gwendolyn K. Kirschner, Elien Vandermarliere, Lennart Martens, Yvonne Stahl, Dominique Audenaert, Jiri Friml, Georg Felix, Ruediger Simon, Malcolm Bennett, Anthony Bishopp, Geert De Jaeger, Karin Ljung, Stefan Kepinski, Stephanie Robert, Jennifer Nemhauser, Ildoo Hwang, Kris Gevaert, Tom Beeckman, Ive De Smet. The CEP5 peptide promotes abiotic stress tolerance, as revealed by quantitative proteomics, and attenuates the AUX/IAA equilibrium in Arabidopsis. J Biol Chem. Year; Vol:pp-pp. © the American Society for Biochemistry and Molecular Biology or © the Author(s).

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