Epidemiological study on prevalence, serovar diversity, multi-drug resistance and CTX-M-type extended-spectrum β-lactamases of Salmonella spp. from patients with diarrhea, food of animal origin, and pets in several provinces of China

Wang, Wei; Zhao, Li; Hu, Yujie; Dottorini, Tania; Fanning, S�amus; Xu, Jin; Li, Fengqin

Abstract

A total of 2,283 Salmonella isolates were recovered from 18,334 samples, including samples from patients with diarrhea, food of animal origin, and pets, across 5 provinces of China. The highest prevalence of Salmonella spp. was detected in chicken meats (39.3%, 486/1,237). Fifteen serogroups and 66 serovars were identified, with Salmonella enterica serovars Typhimurium and Enteritidis being the most dominant. Most (85.5%, 1,952/2,283) isolates exhibited resistance to ≥1 antimicrobial, and 56.4% were multidrug resistant (MDR). A total of 222 isolates harbored extended-spectrum β-lactamases (ESBLs), and 200 of these were of the CTX-M type and were mostly detected in isolates from chicken meat and turtle fecal samples. Overall, eight blaCTX-M genes were identified, with blaCTX-M-65, blaCTX-M-123, blaCTX-M-14, blaCTX-M-79, and blaCTX-M-130 being the most prevalent. In total, 166 of the 222 ESBL-producing isolates had amino acid substitutions in GyrA (S83Y, S83F, D87G, D87N, and D87Y) and ParC (S80I), while the plasmid-mediated quinolone resistance (PMQR)-encoding genes oqxA, oqxB, qepA, qnrB, and qnrS were detected in almost all isolates. Of the 15 sequence types (STs) identified in the 222 ESBLs, ST17, ST11, ST34, and ST26 ranked among the top 5 in number of isolates. Our study revealed considerable serovar diversity and a high prevalence of the co-occurrence of MDR determinants, including CTX-M-type ESBLs, quinolone resistance-determining region (QRDR) mutations, and PMQR genes. This is the first report of CTX-M-130 Salmonella spp. from patients with diarrhea and QRDR mutations from turtle fecal samples. Our study emphasizes the importance of actions, both in health care settings and in the veterinary medicine sector, to control the dissemination of MDR, especially the CTX-M-type ESBL-harboring Salmonella isolates.

Citation

Wang, W., Zhao, L., Hu, Y., Dottorini, T., Fanning, S., Xu, J., & Li, F. (2020). Epidemiological study on prevalence, serovar diversity, multi-drug resistance and CTX-M-type extended-spectrum β-lactamases of Salmonella spp. from patients with diarrhea, food of animal origin, and pets in several provinces of China. Antimicrobial Agents and Chemotherapy, 64(7), Article e00092-20. https://doi.org/10.1128/aac.00092-20

• Journal Article Type: Article
• Acceptance Date: Apr 14, 2020
• Online Publication Date: Apr 20, 2020
• Publication Date: Jun 23, 2020
• Deposit Date: Apr 10, 2021
• Publicly Available Date: Apr 14, 2021
• Journal: Antimicrobial Agents and Chemotherapy
• Print ISSN: 0066-4804
• Electronic ISSN: 1098-6596
• Publisher: American Society for Microbiology
• Peer Reviewed: Peer Reviewed
• Volume: 64
• Issue: 7
• Article Number: e00092-20
• DOI: https://doi.org/10.1128/aac.00092-20
• Keywords: Pharmacology (medical); Pharmacology; Infectious Diseases
• Public URL: https://nottingham-repository.worktribe.com/output/4328153
• Publisher URL: https://aac.asm.org/content/64/7/e00092-20