Skip to main content

Research Repository

Advanced Search

Communication of prostate cancer cells with bone cells via extracellular vesicle RNA: a potential mechanism of metastasis

Probert, C.; Dottorini, T.; Speakman, A.; Hunt, S.; Nafee, T.; Fazeli, A.; Wood, S.; Brown, J.E.; James, V.


C. Probert

A. Speakman

S. Hunt

T. Nafee

A. Fazeli

S. Wood

J.E. Brown


The role of extracellular vesicles (EVs) as vehicles for cell-to-cell communication between a tumour and its environment is a relatively new concept. The hypothesis that EVs may be critical in co-opting tissues by tumours to generate distant metastatic niches is particularly pertinent to prostate cancer (PCa), where metastatic-tropism to bone predominates over other tissue types. The potential role of EVs as a means of communication between prostate cancer cells and cells of the bone stroma such as osteoblasts, is yet to be fully explored.
In this study, we demonstrate that PCa cell EVs both enhance osteoblast viability and produce a significantly more supportive growth environment for PCa cells when grown in co-culture with EV-treated osteoblasts (p less than0.005). Characterisation of the RNA cargo of EVs produced by the bone metastatic PCa cell line PC3, highlights the EV-RNA cargo is significantly enriched in genes relating to cell surface signalling, cell-cell interaction, and protein translation (p less than 0.01). Using novel techniques to track RNA, we demonstrate the delivery of a set of PCa-RNAs to osteoblast via PCa-EVs and show the effect on osteoblast endogenous transcript abundance. Taken together, by using proof-of-concept studies we demonstrate for the first time the contribution of the RNA element of the prostate cancer EV cargo, providing evidence to support PCa EV-communication via RNA molecules as a potential novel route to mediate bone metastasis. We propose targeting prostate cancer EVs could offer a potentially important preventative therapy for men at risk of metastatic prostate cancer.

Journal Article Type Article
Publication Date Oct 23, 2018
Print ISSN 0950-9232
Publisher Nature Publishing Group
Peer Reviewed Peer Reviewed
Volume 38
Pages 1751–1763
APA6 Citation Probert, C., Dottorini, T., Speakman, A., Hunt, S., Nafee, T., Fazeli, A., …James, V. (2018). Communication of prostate cancer cells with bone cells via extracellular vesicle RNA: a potential mechanism of metastasis. Oncogene, 38, 1751–1763. doi:10.1038/s41388-018-0540-5
Publisher URL


You might also like

Downloadable Citations