Covalent co-assembly between resilin-like polypeptide and peptide amphiphile into hydrogels with controlled nanostructure and improved mechanical properties

Okesola, Babatunde O.; Lau, Hang K.; Derkus, Burak; Boccorh, Delali K.; Wu, Yuanhao; Wark, Alastair W.; Kiick, Kristi L.; Mata, Alvaro

Abstract

Covalent co-assembly holds great promise for the fabrication of hydrogels with controllable nanostructure, versatile chemical composition, and enhanced mechanical properties given its relative simplicity, high efficiency, and bond stability. This report describes our approach to designing functional multicomponent hydrogels based on photo-induced chemical interactions between an acrylamide-functionalized resilin-like polypeptide (RLP) and a peptide amphiphile (PA). Circular dichroism (CD) spectroscopy, electron microscopy, and amplitude sweep rheology were used to demonstrate that the co-assembled hydrogel systems acquired distinct structural conformations, tunable nanostructures, and enhanced elasticity in a PA concentration-dependent manner. We envisage the use of these materials in numerous biomedical applications such as controlled drug release systems, microfluidic devices, and scaffolds for tissue engineering.

Citation

Okesola, B. O., Lau, H. K., Derkus, B., Boccorh, D. K., Wu, Y., Wark, A. W., …Mata, A. (2020). Covalent co-assembly between resilin-like polypeptide and peptide amphiphile into hydrogels with controlled nanostructure and improved mechanical properties. Biomaterials Science, 8(3), 846-857. https://doi.org/10.1039/c9bm01796h

• Journal Article Type: Article
• Acceptance Date: Nov 14, 2019
• Online Publication Date: Nov 19, 2019
• Publication Date: Feb 7, 2020
• Deposit Date: Jan 24, 2020
• Publicly Available Date: Nov 20, 2020
• Journal: Biomaterials Science
• Electronic ISSN: 2047-4849
• Publisher: Royal Society of Chemistry
• Peer Reviewed: Peer Reviewed
• Volume: 8
• Issue: 3
• Pages: 846-857
• DOI: https://doi.org/10.1039/c9bm01796h
• Keywords: General Materials Science; Biomedical Engineering
• Public URL: https://nottingham-repository.worktribe.com/output/3793288
• Publisher URL: https://pubs.rsc.org/en/content/articlelanding/2019/bm/c9bm01796h/unauth#!divAbstract
• Additional Information: : This document is Similarity Check deposited; : Supplementary Information; : Babatunde O. Okesola (ORCID); : Babatunde O. Okesola (ResearcherID); : Burak Derkus (ORCID); : Delali K. Boccorh (ORCID); : Alastair W. Wark (ORCID); : Alastair W. Wark (ResearcherID); : Kristi L. Kiick (ORCID); : Alvaro Mata (ORCID); : Single-blind; : Received 5 November 2019; Accepted 14 November 2019; Accepted Manuscript published 19 November 2019; Advance Article published 3 December 2019