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Quantitative phase and polarization imaging through an optical fiber applied to detection of early esophageal tumorigenesis

Gordon, George S. D.; Joseph, James; Alcolea, Maria P.; Sawyer, Travis; Williams, Calum; Fitzpatrick, Catherine R. M.; Jones, Philip H.; Di Pietro, Massimiliano; Fitzgerald, Rebecca C.; Wilkinson, Timothy D.; Bohndiek, Sarah E.

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Authors

James Joseph

Maria P. Alcolea

Travis Sawyer

Calum Williams

Catherine R. M. Fitzpatrick

Philip H. Jones

Massimiliano Di Pietro

Rebecca C. Fitzgerald

Timothy D. Wilkinson

Sarah E. Bohndiek



Abstract

© The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI. Phase and polarization of coherent light are highly perturbed by interaction with microstructural changes in premalignant tissue, holding promise for label-free detection of early tumors in endoscopically accessible tissues such as the gastrointestinal tract. Flexible optical multicore fiber (MCF) bundles used in conventional diagnostic endoscopy and endomicroscopy scramble phase and polarization, restricting clinicians instead to low-contrast amplitude-only imaging. We apply a transmission matrix characterization approach to produce full-field en-face images of amplitude, quantitative phase, and resolved polarimetric properties through an MCF. We first demonstrate imaging and quantification of biologically relevant amounts of optical scattering and birefringence in tissue-mimicking phantoms. We present an entropy metric that enables imaging of phase heterogeneity, indicative of disordered tissue microstructure associated with early tumors. Finally, we demonstrate that the spatial distribution of phase and polarization information enables label-free visualization of early tumors in esophageal mouse tissues, which are not identifiable using conventional amplitude-only information.

Citation

Gordon, G. S. D., Joseph, J., Alcolea, M. P., Sawyer, T., Williams, C., Fitzpatrick, C. R. M., …Bohndiek, S. E. (2019). Quantitative phase and polarization imaging through an optical fiber applied to detection of early esophageal tumorigenesis. Journal of Biomedical Optics, 24(12), Article 126004. https://doi.org/10.1117/1.JBO.24.12.126004

Journal Article Type Article
Acceptance Date Nov 18, 2019
Online Publication Date Dec 16, 2019
Publication Date Dec 16, 2019
Deposit Date Jan 22, 2020
Publicly Available Date Mar 29, 2024
Journal Journal of Biomedical Optics
Print ISSN 1083-3668
Electronic ISSN 1560-2281
Publisher Society of Photo-optical Instrumentation Engineers
Peer Reviewed Peer Reviewed
Volume 24
Issue 12
Article Number 126004
DOI https://doi.org/10.1117/1.JBO.24.12.126004
Keywords Atomic and Molecular Physics, and Optics; Electronic, Optical and Magnetic Materials; Biomaterials; Biomedical Engineering
Public URL https://nottingham-repository.worktribe.com/output/3780941
Publisher URL https://www.spiedigitallibrary.org/journals/journal-of-biomedical-optics/volume-24/issue-12/126004/Quantitative-phase-and-polarization-imaging-through-an-optical-fiber-applied/10.1117/1.JBO.24.12.126004.full?SSO=1

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