Sulfadiazine Sodium Ameliorates the Metabolomic Perturbation in Mice Infected with Toxoplasma gondii

Zhu, Xing-Quan; Zhou, Chun-Xue; Liu, Qing; Gan, Yun; Cong, Hua; Elsheikha, Hany M; Chen, Xiao-Qing

Abstract

In this study, we analyzed the global metabolomic changes associated with Toxoplasma gondii infection in mice in the presence or absence of sulfadiazine sodium (SDZ) treatment. BALB/c mice were infected with T. gondii GT1 strain and treated orally with SDZ (250 g/ml in water) for 12 consecutive days. Mice showed typical manifestations of illness at 20 days postinfection (dpi); by 30 dpi, 20% had survived and developed latent infection. We used ultraperformance liquid chromatography-mass spectrometry to profile the serum metabolomes in control (untreated and uninfected) mice, acutely infected mice, and SDZ-treated and infected mice. Infection induced significant perturbations in the metabolism of-linolenic acid, purine, pyrimidine, arginine, tryptophan, valine, glycerophospholipids, and fatty acyls. However, treatment with SDZ seemed to alleviate the serum metabolic alterations caused by infection. The restoration of the serum metabolite levels in the treated mice was associated with better clinical outcomes. These data indicate that untargeted metabolomics can reveal biochemical pathways associated with restoration of the metabolic status of T. gondii-infected mice following SDZ treatment and could be used to monitor responses to SDZ treatment. This study provides a new systems approach to elucidate the metabolic and therapeutic effects of SDZ in the context of murine toxoplasmosis. K E Y W O R D S Toxoplasma gondii, biomarkers, metabolomics, mice, serum metabolites, sulfadiazine sodium Toxoplasma gondii, an obligate intracellular protozoan parasite, is highly prevalent in warm-blooded animals and humans (1). T. gondii comprises three clonal lineages (type I, type II, and type III) (2). Despite 98% genetic similarity, dramatic differences in virulence exist among strains belonging to these T. gondii genotypes (3). Humans acquire infection mainly by ingesting undercooked meat containing tissue cysts or oocysts from contaminated water (4). Acute infection with this parasite is mediated by the aggressive, fast-replicating, tachyzoite stage, which can cause encephalitis or retinochoroiditis. In addition, reactivation of the latent form (i.e., bradyzoites-containing cysts) of T. gondii can cause life-threatening conditions and even death in immuno-compromised individuals (5).

Citation

Zhu, X.-Q., Zhou, C.-X., Liu, Q., Gan, Y., Cong, H., Elsheikha, H. M., & Chen, X.-Q. (2019). Sulfadiazine Sodium Ameliorates the Metabolomic Perturbation in Mice Infected with Toxoplasma gondii. Antimicrobial Agents and Chemotherapy, 63(10), Article e00312-19. https://doi.org/10.1128/AAC.00312-19

• Journal Article Type: Article
• Acceptance Date: Jul 25, 2019
• Online Publication Date: Aug 5, 2019
• Publication Date: Sep 23, 2019
• Deposit Date: Sep 22, 2020
• Publicly Available Date: Sep 24, 2020
• Journal: Antimicrobial Agents and Chemotherapy
• Print ISSN: 0066-4804
• Electronic ISSN: 1098-6596
• Publisher: American Society for Microbiology
• Peer Reviewed: Peer Reviewed
• Volume: 63
• Issue: 10
• Article Number: e00312-19
• DOI: https://doi.org/10.1128/AAC.00312-19
• Public URL: https://nottingham-repository.worktribe.com/output/3629896
• Publisher URL: https://aac.asm.org/content/63/10/e00312-19