Growth and quantification of MERS-CoV infection

Coleman, Christopher M.; Frieman, Matthew B.

Abstract

© 2015 by John Wiley & Sons, Inc. Middle East respiratory syndrome coronavirus (MERS-CoV) is an emerging highly pathogenic respiratory virus. Although MERS-CoV only emerged in 2012, we and others have developed assays to grow and quantify infectious MERS-CoV and RNA products of replication in vitro. MERS-CoV is able to infect a range of cell types, but replicates to high titers in Vero E6 cells. Protocols for the propagation and quantification of MERS-CoV are presented.

Citation

Coleman, C. M., & Frieman, M. B. (2015). Growth and quantification of MERS-CoV infection. Current Protocols in Microbiology, 37(1), 15E.2.1-15E.2.9. https://doi.org/10.1002/9780471729259.mc15e02s37

• Journal Article Type: Article
• Acceptance Date: May 1, 2015
• Online Publication Date: May 1, 2015
• Publication Date: May 1, 2015
• Deposit Date: Dec 17, 2019
• Journal: Current Protocols in Microbiology
• Print ISSN: 1934-8525
• Electronic ISSN: 1934-8533
• Publisher: Wiley
• Peer Reviewed: Not Peer Reviewed
• Volume: 37
• Issue: 1
• Pages: 15E.2.1-15E.2.9
• DOI: https://doi.org/10.1002/9780471729259.mc15e02s37
• Public URL: https://nottingham-repository.worktribe.com/output/3590065