The chemotrophic behaviour of Aspergillus niger: Mapping hyphal filaments during chemo-sensing; the first step towards directed materials formation

Sadaf, Ayesha; Brock, Matthias; Perry, Carole C.

Abstract

In the development of fungal based materials for applications in construction through to biomedical materials and fashion, understanding how to regulate and direct growth is key for gaining control over the form of material generated. Here, we show how simple ‘chemical food’ cues can be used to manipulate the growth of fungal networks by taking Aspergillus niger as an exemplar species. Chemotrophic responses towards a range of nitrogen and carbon containing biomolecules including amino acids, sugars and sugar alcohols were quantified in terms of chemotrophic index (CI) under a range of basal media compositions (low and high concentrations of N and C sources). Growth of filamentous networks was followed using fluorescence microscopy at single time points and during growth by an AI analytical approach to explore chemo sensing behaviour of the fungus when exposed to pairs (C-C, C-N, N-N) of biomolecules simultaneously. Data suggests that the directive growth of A. niger can be controlled towards simple biomolecules with CI values giving a good approximation for expected growth under a range of growth conditions. This is a first step towards identifying conditions for researcher-led directed growth of hyphae to make mycelial mats with tuneable morphological, physicochemical, and mechanical characteristics.

Citation

Sadaf, A., Brock, M., & Perry, C. C. (2024). The chemotrophic behaviour of Aspergillus niger: Mapping hyphal filaments during chemo-sensing; the first step towards directed materials formation. Fungal Biology, 128(4), 1868-1875. https://doi.org/10.1016/j.funbio.2024.05.010

• Journal Article Type: Article
• Acceptance Date: May 24, 2024
• Online Publication Date: May 25, 2024
• Publication Date: Jun 1, 2024
• Deposit Date: Oct 4, 2024
• Publicly Available Date: Oct 9, 2024
• Journal: Fungal Biology
• Print ISSN: 1878-6146
• Electronic ISSN: 1878-6162
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 128
• Issue: 4
• Pages: 1868-1875
• DOI: https://doi.org/10.1016/j.funbio.2024.05.010
• Public URL: https://nottingham-repository.worktribe.com/output/35712620
• Publisher URL: https://www.sciencedirect.com/science/article/pii/S1878614624000692?via%3Dihub