The cytoskeletal scaffold Shank3 is recruited to pathogen-induced actin rearrangements

Huett, Alan; Leong, John M.; Podolsky, Daniel K.; Xavier, Ramnik J.

Abstract

The common gastrointestinal pathogens enteropathogenic Escherichia coli (EPEC) and Salmonella typhimurium both reorganize the gut epithelial cell actin cytoskeleton to mediate pathogenesis, utilizing mimicry of the host signaling apparatus. The PDZ domain-containing protein Shank3, is a large cytoskeletal scaffold protein with known functions in neuronal morphology and synaptic signaling, and is also capable of acting as a scaffolding adaptor during Ret tyrosine kinase signaling in epithelial cells. Using immunofluorescent and functional RNA-interference approaches we show that Shank3 is present in both EPEC- and S. typhimurium-induced actin rearrangements and is required for optimal EPEC pedestal formation. We propose that Shank3 is one of a number of host synaptic proteins likely to play key roles in bacteria-host interactions. © 2009 Elsevier Inc. All rights reserved.

Citation

Huett, A., Leong, J. M., Podolsky, D. K., & Xavier, R. J. (2009). The cytoskeletal scaffold Shank3 is recruited to pathogen-induced actin rearrangements. Experimental Cell Research, 315(12), 2001-2011. https://doi.org/10.1016/j.yexcr.2009.04.003

• Journal Article Type: Article
• Acceptance Date: Apr 6, 2009
• Online Publication Date: Apr 14, 2009
• Publication Date: Jul 15, 2009
• Deposit Date: Aug 15, 2022
• Journal: Experimental Cell Research
• Print ISSN: 0014-4827
• Electronic ISSN: 1090-2422
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 315
• Issue: 12
• Pages: 2001-2011
• DOI: https://doi.org/10.1016/j.yexcr.2009.04.003
• Public URL: https://nottingham-repository.worktribe.com/output/3181812
• Publisher URL: https://www.sciencedirect.com/science/article/pii/S0014482709001542?via%3Dihub