Dr NEIL OLDFIELD NEIL.OLDFIELD@NOTTINGHAM.AC.UK
ASSISTANT PROFESSOR
T-cell stimulating protein A (TspA) of Neisseria meningitidis is required for optimal adhesion to human cells
Oldfield, Neil J.; Bland, Sarah J.; Taraktsoglou, Maria; Dos Ramos, Francisco J.; Robinson, Karen; Wooldridge, Karl G.; Ala'Aldeen, Dlawer A.A.
Authors
Sarah J. Bland
Maria Taraktsoglou
Francisco J. Dos Ramos
Professor KAREN ROBINSON karen.robinson@nottingham.ac.uk
PROFESSOR IN GASTROINTESTINAL INFECTION AND IMMUNITY
Dr KARL WOOLDRIDGE KARL.WOOLDRIDGE@NOTTINGHAM.AC.UK
ASSOCIATE PROFESSOR
Dlawer A.A. Ala'Aldeen
Abstract
T-cell stimulating protein A (TspA) is an immunogenic, T-cell and B-cell stimulating protein of Neisseria meningitidis. Sequence similarity between TspA and FimV, a Pseudomonas aeruginosa protein involved in twitching motility, suggested a link between TspA and type IV pili (Tfp). To determine the role of TspA an isogenic deletion mutant was created. Loss of TspA did not affect twitching motility or piliation indicating that there are functional differences between TspA and FimV. Mutation of tspA led to a significant reduction in adhesion of meningococci to meningothelial and HEp-2 cells, which was not due to a lack of transcription of adjacent genes or pilC1. Other Tfp-mediated phenotypes (i.e. auto-aggregation and transformation competence) were not altered. Our results indicate that the role of TspA in adhesion is unlikely to be directly linked to the function of Tfp. TspA was expressed by all N.meningitidis and Neisseria polysaccharea strains examined but not by Neisseria gonorrhoeae or Neisseria lactamica, although sequences with homology to tspA were present in their genomes. In summary, TspA is a highly conserved antigen that is required for optimal adhesion of meningococci to human cells. © 2006 The Authors; Journal compilation © 2006 Blackwell Publishing Ltd.
Citation
Oldfield, N. J., Bland, S. J., Taraktsoglou, M., Dos Ramos, F. J., Robinson, K., Wooldridge, K. G., & Ala'Aldeen, D. A. (2007). T-cell stimulating protein A (TspA) of Neisseria meningitidis is required for optimal adhesion to human cells. Cellular Microbiology, 9(2), 463-478. https://doi.org/10.1111/j.1462-5822.2006.00803.x
Journal Article Type | Article |
---|---|
Acceptance Date | Jul 11, 2007 |
Online Publication Date | Sep 11, 2006 |
Publication Date | 2007-02 |
Deposit Date | Aug 2, 2022 |
Journal | Cellular Microbiology |
Print ISSN | 1462-5814 |
Electronic ISSN | 1462-5822 |
Publisher | Wiley |
Peer Reviewed | Peer Reviewed |
Volume | 9 |
Issue | 2 |
Pages | 463-478 |
DOI | https://doi.org/10.1111/j.1462-5822.2006.00803.x |
Public URL | https://nottingham-repository.worktribe.com/output/3129935 |
Publisher URL | https://onlinelibrary.wiley.com/doi/10.1111/j.1462-5822.2006.00803.x |
You might also like
Downloadable Citations
About Repository@Nottingham
Administrator e-mail: discovery-access-systems@nottingham.ac.uk
This application uses the following open-source libraries:
SheetJS Community Edition
Apache License Version 2.0 (http://www.apache.org/licenses/)
PDF.js
Apache License Version 2.0 (http://www.apache.org/licenses/)
Font Awesome
SIL OFL 1.1 (http://scripts.sil.org/OFL)
MIT License (http://opensource.org/licenses/mit-license.html)
CC BY 3.0 ( http://creativecommons.org/licenses/by/3.0/)
Powered by Worktribe © 2025
Advanced Search