James F. E. Grey
The use of decellularised animal tissue to study disseminating cancer cells
Grey, James F. E.; Campbell-Ritchie, Alastair; Everitt, Nicola M.; Fezovich, Alexander J.; Wheatley, Sally P.
Authors
ALASTAIR CAMPBELL RITCHIE alastair.campbellritchie@nottingham.ac.uk
Assistant Professor
Nicola M. Everitt
Alexander J. Fezovich
SALLY WHEATLEY sally.wheatley@nottingham.ac.uk
Assistant Professor
Abstract
Since the establishment of cell culture, common practice has been to grow adherent cells in 2D monolayers. Although cells behave completely differently when grown under these artificial conditions, the ease of 2D culturing has meant that this practice still prevails, and adopting conditions that more closely reflect the natural microenvironment has been met with substantial inertia. The alternative, animal models that mimic natural human physiology, are less accessible, strictly regulated and require licences and expensive facilities. Although transition from 2D to 3D cell culturing is gathering momentum, there is a clear need for alternative culturing methods that more closely resemble in vivo conditions. Here, we show that decellularised organs gleaned from discarded animal carcasses are ideal biomimetic scaffolds to support secondary tumour initiation in vitro. Further, we describe how to decellularise tissue and perform basic histochemistry and immunofluorescence procedures for cell and matrix detection. Cancer cell behaviour on this matrix is followed by way of an example. Because integration into the traditional work flow is easy and inexpensive, we hope this article will encourage other researchers to adopt this approach.
Citation
Grey, J. F. E., Campbell-Ritchie, A., Everitt, N. M., Fezovich, A. J., & Wheatley, S. P. (2019). The use of decellularised animal tissue to study disseminating cancer cells. Journal of Cell Science, 132(4), Article jcs219907. https://doi.org/10.1242/jcs.219907
Journal Article Type | Article |
---|---|
Acceptance Date | Oct 15, 2018 |
Online Publication Date | Dec 10, 2018 |
Publication Date | Feb 15, 2019 |
Deposit Date | Apr 27, 2020 |
Publicly Available Date | Apr 27, 2020 |
Journal | Journal of Cell Science |
Print ISSN | 0021-9533 |
Electronic ISSN | 1477-9137 |
Publisher | Company of Biologists |
Peer Reviewed | Peer Reviewed |
Volume | 132 |
Issue | 4 |
Article Number | jcs219907 |
DOI | https://doi.org/10.1242/jcs.219907 |
Public URL | https://nottingham-repository.worktribe.com/output/3017607 |
Publisher URL | https://jcs.biologists.org/content/132/4/jcs219907 |
Files
jcs219907.full
(2.3 Mb)
PDF
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