Immobilized Enzymes on Gold Nanoparticles: From Enhanced Stability to Cleaning of Heritage Textiles
Gherardi, Francesca; Turyanska, Lyudmila; Ferrari, Enrico; Weston, Nicola; Fay, Michael W.; Colston, Belinda Jayne
Dr MICHAEL FAY MICHAEL.FAY@NOTTINGHAM.AC.UK
Senior Research Fellow
Belinda Jayne Colston
Enzyme-based treatments are used in heritage conservation for the effective removal of glues and other damaging organic layers from the surfaces of historic and artistic works. Despite their potential, however, the application of enzymatic treatments is currently limited because of their poor efficiency and low operational and environmental stability. We demonstrate the use of α-amylase immobilized on gold nanoparticles to improve the efficacy of enzymatic treatments enhancing both the reactivity and the stability of the formulations. Gold nanoparticles coated with α-amylase exhibit significant advantages compared to free enzymes. We report up to 5× greater resistance to environmental changes, up to 2× higher efficacy toward removal of starch-based glues from textiles and deeper penetration through the fibers, without causing damage or inducing salt precipitation. These results offer exciting prospects for the development of enzymatic formulations, both for heritage conservation and the wider application of enzymes, such as in medicine, the detergent industry, and green chemistry.
|Journal Article Type||Article|
|Publication Date||Nov 18, 2019|
|Journal||ACS Applied Bio Materials|
|Publisher||American Chemical Society|
|Peer Reviewed||Peer Reviewed|
|APA6 Citation||Gherardi, F., Turyanska, L., Ferrari, E., Weston, N., Fay, M. W., & Colston, B. J. (2019). Immobilized Enzymes on Gold Nanoparticles: From Enhanced Stability to Cleaning of Heritage Textiles. ACS Applied Bio Materials, 2(11), 5136-5143. https://doi.org/10.1021/acsabm.9b00802|
|Keywords||Gold nanoparticles; Amylase; Textiles; Starch cleaning; Cultural heritage|
This file is under embargo until Oct 22, 2020 due to copyright restrictions.
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