Linking long-term soil phosphorus management to microbial communities involved in nitrogen reactions

O’Neill, R. M.; Duff, A. M.; Brennan, F. P.; Gebremichael, A. W.; Girkin, N. T.; Lanigan, G. J.; Krol, D. J.; Wall, D. P.; Renou-Wilson, F.; Müller, C.; Richards, K. G.; Deveautour, C.

Abstract

The influence of soil phosphorous (P) content on the N-cycling communities and subsequent effects on N2O emissions remains unclear. Two laboratory incubation experiments were conducted on soils collected from a long-term (est. 1995) P-addition field trial sampled in summer 2018 and winter 2019. Incubations were treated with a typical field amendment rate of N as well as a C-amendment to stimulate microbial activity. Throughout both incubations, soil subsamples were collected prior to fertiliser amendment and then throughout the incubations, to quantify the abundance of bacteria (16S rRNA), fungi (ITS) and Thaumarcheota (16S rRNA) as well as functional guilds of genes involved in nitrification (bacterial and archaeal amoA, and comammox) and denitrification (nirS, nirK, nosZ clade I and II) using quantitative PCR (qPCR). We also evaluated the correlations between each gene abundance and the associated N2O emissions depending on P-treatments. Our results show that long-term P-application influenced N-cycling genes abundance differently. Except for comammox, overall nitrifiers’ genes were most abundant in low P while the opposite trend was found for denitrifiers’ genes. C and N-amendments strongly influenced the abundance of most genes with changes observed as soon as 24 h after application. ITS was the only gene correlated to N2O emissions in the low P-soils while microbes were mostly correlated to emissions in high P, suggesting possible changes in the organisms involved in N2O production depending on soil P-content. This study highlights the importance of long-term P addition on shaping the microbial community function which in turn stimulates a direct impact on the subsequent N emissions.

Citation

O’Neill, R. M., Duff, A. M., Brennan, F. P., Gebremichael, A. W., Girkin, N. T., Lanigan, G. J., Krol, D. J., Wall, D. P., Renou-Wilson, F., Müller, C., Richards, K. G., & Deveautour, C. (2022). Linking long-term soil phosphorus management to microbial communities involved in nitrogen reactions. Biology and Fertility of Soils, 58(4), 389-402. https://doi.org/10.1007/s00374-022-01627-y

• Journal Article Type: Article
• Acceptance Date: Feb 9, 2022
• Online Publication Date: Feb 24, 2022
• Publication Date: 2022-05
• Deposit Date: Jan 13, 2024
• Publicly Available Date: Jan 16, 2024
• Journal: Biology and Fertility of Soils
• Print ISSN: 0178-2762
• Electronic ISSN: 1432-0789
• Publisher: Springer Verlag
• Peer Reviewed: Peer Reviewed
• Volume: 58
• Issue: 4
• Pages: 389-402
• DOI: https://doi.org/10.1007/s00374-022-01627-y
• Keywords: Functional genes; Nitrifiers · Denitrifiers · Fungi · qPCR · Phosphorous · Nitrous oxide
• Public URL: https://nottingham-repository.worktribe.com/output/29584308
• Publisher URL: https://link.springer.com/article/10.1007/s00374-022-01627-y
• Additional Information: Received: 4 October 2021; Revised: 8 February 2022; Accepted: 9 February 2022; First Online: 24 February 2022; : ; : The authors declare no competing interests.