Pharmacological profile of vascular activity of human stem villous arteries

Sander, Katrin N.; Ali, Tayyba Y.; Warren, Averil Y.; Hay, Daniel P.; Broughton Pipkin, Fiona; Barrett, David A.; Khan, Raheela N.

Abstract

© 2019 Introduction: The function of the placental vasculature differs considerably from other systemic vascular beds of the human body. A detailed understanding of the normal placental vascular physiology is the foundation to understand perturbed conditions potentially leading to placental dysfunction. Methods: Behaviour of human stem villous arteries isolated from placentae at term pregnancy was assessed using wire myography. Effects of a selection of known vasoconstrictors and vasodilators of the systemic vasculature were assessed. The morphology of stem villous arteries was examined using IHC and TEM. Results: Contractile effects in stem villous arteries were caused by U46619, 5-HT, angiotensin II and endothelin-1 (p ≤ 0.05), whereas noradrenaline and AVP failed to result in a contraction. Dilating effects were seen for histamine, riluzole, nifedipine, papaverine, SNP and SQ29548 (p ≤ 0.05) but not for acetylcholine, bradykinin and substance P. Discussion: Stem villous arteries behave differently to vessels of the systemic vasculature and results indicate that the placenta is cut off from the systemic maternal vascular regulation. Particularly, endothelium-dependent processes were attenuated in the placental vasculature, creating a need to determine the role of the endothelium in the placenta in future studies.

Citation

Sander, K. N., Ali, T. Y., Warren, A. Y., Hay, D. P., Broughton Pipkin, F., Barrett, D. A., & Khan, R. N. (2019). Pharmacological profile of vascular activity of human stem villous arteries. Placenta, 88, 12-19. https://doi.org/10.1016/j.placenta.2019.09.009

• Journal Article Type: Article
• Acceptance Date: Sep 16, 2019
• Online Publication Date: Sep 17, 2019
• Publication Date: 2019-12
• Deposit Date: Sep 30, 2019
• Publicly Available Date: Sep 18, 2020
• Journal: Placenta
• Print ISSN: 0143-4004
• Electronic ISSN: 1532-3102
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 88
• Pages: 12-19
• DOI: https://doi.org/10.1016/j.placenta.2019.09.009
• Keywords: Obstetrics and Gynaecology; Developmental Biology; Reproductive Medicine
• Public URL: https://nottingham-repository.worktribe.com/output/2722958
• Publisher URL: https://www.sciencedirect.com/science/article/pii/S0143400419306678
• Additional Information: This article is maintained by: Elsevier; Article Title: Pharmacological profile of vascular activity of human stem villous arteries; Journal Title: Placenta; CrossRef DOI link to publisher maintained version: https://doi.org/10.1016/j.placenta.2019.09.009; Content Type: article; Copyright: © 2019 Published by Elsevier Ltd.
• Contract Date: Sep 30, 2019