Osteocalcin does not influence acute or chronic inflammation in human vascular cells

Millar, Sophie A.; Zala, Ieva; Anderson, Susan I.; O'Sullivan, Saoirse E.

Abstract

Some human observational studies have suggested an anti-inflammatory role of osteocalcin (OCN). An inflammatory protocol using IFN-γ and TNF-α (10 ng/mL) was employed to examine the acute (24 hr) and chronic (144 hr) effects of uncarboxylated OCN (ucOCN) in commercial, primary, sub-cultured human aortic endothelial (HAEC) and smooth muscle cells (HASMCs). The inflammatory protocol increased phosphorylation of intracellular signalling proteins (CREB, JNK, p38, ERK, AKT, STAT3, STAT5) and increased secretion of adhesion markers (VCAM-1, ICAM-1, MCP-1) and pro-inflammatory cytokines (IL-6, IL-8). After acute inflammation, there were no additive or reductive effects of ucOCN in either cell type. Following chronic inflammation, ucOCN did not affect cell responses, nor did it appear to have any pro- or anti-inflammatory effects when administered acutely or chronically on its own in either cell type. Additionally, ucOCN did not affect lipopolysaccharide (LPS) induced acute inflammation in HAECs or HASMCs. The findings of this study do not support a causal role for OCN within the models of vascular inflammation chosen. Further confirmatory studies are warranted.

Citation

Millar, S. A., Zala, I., Anderson, S. I., & O'Sullivan, S. E. (2020). Osteocalcin does not influence acute or chronic inflammation in human vascular cells. Journal of Cellular Physiology, 235(4), 3414-3424. https://doi.org/10.1002/jcp.29231

• Journal Article Type: Article
• Acceptance Date: Sep 4, 2019
• Online Publication Date: Sep 24, 2019
• Publication Date: 2020-04
• Deposit Date: Sep 10, 2019
• Publicly Available Date: Sep 25, 2020
• Journal: Journal of Cellular Physiology
• Print ISSN: 0021-9541
• Electronic ISSN: 1097-4652
• Publisher: Wiley
• Peer Reviewed: Peer Reviewed
• Volume: 235
• Issue: 4
• Pages: 3414-3424
• DOI: https://doi.org/10.1002/jcp.29231
• Keywords: Osteocalcin, Inflammation, Endothelial, Smooth muscle, Bone hormone
• Public URL: https://nottingham-repository.worktribe.com/output/2595929
• Publisher URL: https://onlinelibrary.wiley.com/doi/full/10.1002/jcp.29231
• Additional Information: Received: 2019-04-25; Accepted: 2019-09-04; Published: 2019-09-24