Skip to main content

Research Repository

Advanced Search

Osteocalcin does not influence acute or chronic inflammation in human vascular cells

Millar, Sophie A.; Zala, Ieva; Anderson, Susan I.; O'Sullivan, Saoirse E.


Sophie A. Millar

Ieva Zala

Saoirse E. O'Sullivan


Some human observational studies have suggested an anti-inflammatory role of osteocalcin (OCN). An inflammatory protocol using IFN-? and TNF-? (10 ng/mL) was employed to examine the acute (24 hr) and chronic (144 hr) effects of uncarboxylated OCN (ucOCN) in commercial, primary, sub-cultured human aortic endothelial (HAEC) and smooth muscle cells (HASMCs). The inflammatory protocol increased phosphorylation of intracellular signalling proteins (CREB, JNK, p38, ERK, AKT, STAT3, STAT5) and increased secretion of adhesion markers (VCAM-1, ICAM-1, MCP-1) and pro-inflammatory cytokines (IL-6, IL-8). After acute inflammation, there were no additive or reductive effects of ucOCN in either cell type. Following chronic inflammation, ucOCN did not affect cell responses, nor did it appear to have any pro- or anti-inflammatory effects when administered acutely or chronically on its own in either cell type. Additionally, ucOCN did not affect lipopolysaccharide (LPS) induced acute inflammation in HAECs or HASMCs. The findings of this study do not support a causal role for OCN within the models of vascular inflammation chosen. Further confirmatory studies are warranted.


Millar, S. A., Zala, I., Anderson, S. I., & O'Sullivan, S. E. (2020). Osteocalcin does not influence acute or chronic inflammation in human vascular cells. Journal of Cellular Physiology, 235(4), 3414-3424.

Journal Article Type Article
Acceptance Date Sep 4, 2019
Online Publication Date Sep 24, 2019
Publication Date 2020-04
Deposit Date Sep 10, 2019
Publicly Available Date Sep 25, 2020
Journal Journal of Cellular Physiology
Print ISSN 0021-9541
Electronic ISSN 1097-4652
Publisher Wiley
Peer Reviewed Peer Reviewed
Volume 235
Issue 4
Pages 3414-3424
Keywords Osteocalcin, Inflammation, Endothelial, Smooth muscle, Bone hormone
Public URL
Publisher URL
Additional Information Received: 2019-04-25; Accepted: 2019-09-04; Published: 2019-09-24


You might also like

Downloadable Citations