Bioconversion of underutilized brewing by-products into bacterial cellulose by a newly isolated Komagataeibacter rhaeticus strain: A preliminary evaluation of the bioprocess environmental impact

Tsouko, Erminta; Pilafidis, Sotiris; Dimopoulou, Maria; Kourmentza, Konstantina; Sarris, Dimitris

Abstract

A novel Komagataeibacter rhaeticus UNIWA AAK2 strain was used to produce bacterial cellulose (BC), valorizing brewers’ spent grain (BSG) and brewer’s spent yeast (BSY). Under optimal conditions (controlled pH = 6 and 30 g/L sugars), a maximum BC of 4.0 g/L was achieved when BSG aqueous extract (BSGE) was used. The substitution of yeast extract and peptone with BSY autolyzates did not show significant differences on BC concentration and productivity. The FTIR, SEM, and TGA analyses showed that the use of brewing by-products had no effect on the structure and thermal stability of the produced BC, compared to highly-pure and commercial substrates. The LCA of the developed bioprocess revealed that BSGE- and BSY-based media can reduce the carbon footprint of 1 kg dry BC by 76% compared to commercial-based-media. Beer by-products could serve as cost-effective resources to produce value-added and sustainable biopolymers such as BC, while minimizing waste and restructuring the brewing-industry.

Citation

Tsouko, E., Pilafidis, S., Dimopoulou, M., Kourmentza, K., & Sarris, D. (2023). Bioconversion of underutilized brewing by-products into bacterial cellulose by a newly isolated Komagataeibacter rhaeticus strain: A preliminary evaluation of the bioprocess environmental impact. Bioresource Technology, 387, Article 129667. https://doi.org/10.1016/j.biortech.2023.129667

• Journal Article Type: Article
• Acceptance Date: Aug 9, 2023
• Online Publication Date: Aug 10, 2023
• Publication Date: 2023-11
• Deposit Date: Sep 7, 2023
• Publicly Available Date: Aug 11, 2024
• Journal: Bioresource Technology
• Print ISSN: 0960-8524
• Electronic ISSN: 1873-2976
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 387
• Article Number: 129667
• DOI: https://doi.org/10.1016/j.biortech.2023.129667
• Keywords: Waste Management and Disposal; Renewable Energy, Sustainability and the Environment; General Medicine; Environmental Engineering; Bioengineering
• Public URL: https://nottingham-repository.worktribe.com/output/24148238
• Publisher URL: https://www.sciencedirect.com/science/article/abs/pii/S0960852423010957?via%3Dihub
• Additional Information: This article is maintained by: Elsevier; Article Title: Bioconversion of underutilized brewing by-products into bacterial cellulose by a newly isolated Komagataeibacter rhaeticus strain: A preliminary evaluation of the bioprocess’s environmental impact; Journal Title: Bioresource Technology; CrossRef DOI link to publisher maintained version: https://doi.org/10.1016/j.biortech.2023.129667; Content Type: article; Copyright: © 2023 Elsevier Ltd. All rights reserved.