A sustainable bioprocess to produce bacterial cellulose (BC) using waste streams from wine distilleries and the biodiesel industry: evaluation of BC for adsorption of phenolic compounds, dyes and metals

Tsouko, Erminta; Pilafidis, Sotirios; Kourmentza, Konstantina; Gomes, Helena I.; Sarris, Giannis; Koralli, Panagiota; Papagiannopoulos, Aristeidis; Pispas, Stergios; Sarris, Dimitris

Abstract

Background
The main challenge for large-scale production of bacterial cellulose (BC) includes high production costs interlinked with raw materials, and low production rates. The valorization of renewable nutrient sources could improve the economic effectiveness of BC fermentation while their direct bioconversion into sustainable biopolymers addresses environmental pollution and/or resource depletion challenges. Herein a green bioprocess was developed to produce BC in high amounts with the rather unexplored bacterial strain Komagataeibacter rhaeticus, using waste streams such as wine distillery effluents (WDE) and biodiesel-derived glycerol. Also, BC was evaluated as a bio-adsorbent for phenolics, dyes and metals removal to enlarge its market diversification.

Results
BC production was significantly affected by the WDE mixing ratio (0–100%), glycerol concentration (20–45 g/L), type of glycerol and media-sterilization method. A maximum BC concentration of 9.0 g/L, with a productivity of 0.90 g/L/day and a water holding capacity of 60.1 g water/g dry BC, was achieved at 100% WDE and ≈30 g/L crude glycerol. BC samples showed typical cellulose vibration bands and average fiber diameters between 37.2 and 89.6 nm. The BC capacity to dephenolize WDE and adsorb phenolics during fermentation reached respectively, up to 50.7% and 26.96 mg gallic acid equivalents/g dry BC (in-situ process). The produced BC was also investigated for dye and metal removal. The highest removal of dye acid yellow 17 (54.3%) was recorded when 5% of BC was applied as the bio-adsorbent. Experiments performed in a multi-metal synthetic wastewater showed that BC could remove up to 96% of Zn and 97% of Cd.

Conclusions
This work demonstrated a low-carbon approach to produce low-cost, green and biodegradable BC-based bio-adsorbents, without any chemical modification. Their potential in wastewater-treatment-applications was highlighted, promoting closed-loop systems within the circular economy era. This study may serve as an orientation for future research towards competitive or targeted adsorption technologies for wastewater treatment or resources recovery.

Citation

Tsouko, E., Pilafidis, S., Kourmentza, K., Gomes, H. I., Sarris, G., Koralli, P., …Sarris, D. (2024). A sustainable bioprocess to produce bacterial cellulose (BC) using waste streams from wine distilleries and the biodiesel industry: evaluation of BC for adsorption of phenolic compounds, dyes and metals. Biotechnology for Biofuels and Bioproducts, 17(1), Article 40. https://doi.org/10.1186/s13068-024-02488-3

• Journal Article Type: Article
• Acceptance Date: Mar 4, 2024
• Online Publication Date: Mar 12, 2024
• Publication Date: 2024
• Deposit Date: Mar 13, 2024
• Publicly Available Date: Mar 14, 2024
• Journal: Biotechnology for Biofuels and Bioproducts
• Electronic ISSN: 2731-3654
• Publisher: BioMed Central
• Peer Reviewed: Peer Reviewed
• Volume: 17
• Issue: 1
• Article Number: 40
• DOI: https://doi.org/10.1186/s13068-024-02488-3
• Keywords: Low-cost feedstock, Dephenolization, Wastewater treatment, Wine industry, BC-based adsorbent, Heavy metals
• Public URL: https://nottingham-repository.worktribe.com/output/32460119
• Publisher URL: https://biotechnologyforbiofuels.biomedcentral.com/articles/10.1186/s13068-024-02488-3