Relationship between neuromelanin and dopamine terminals within the Parkinson’s nigrostriatal system

Mart�n-Bastida, Antonio; Lao-Kaim, Nicholas P.; Roussakis, Andreas Antonios; Searle, Graham E.; Xing, Yue; Gunn, Roger N.; Schwarz, Stefan T.; Barker, Roger A.; Auer, Dorothee P.; Piccini, Paola

Abstract

© 2019 The Author(s). All rights reserved. Parkinson's disease is characterized by the progressive loss of pigmented dopaminergic neurons in the substantia nigra and associated striatal deafferentation. Neuromelanin content is thought to reflect the loss of pigmented neurons, but available data characterizing its relationship with striatal dopaminergic integrity are not comprehensive or consistent, and predominantly involve heterogeneous samples. In this cross-sectional study, we used neuromelanin-sensitive MRI and the highly specific dopamine transporter PET radioligand, 11C-PE2I, to assess the association between neuromelanin-containing cell levels in the substantia nigra pars compacta and nigrostriatal terminal density in vivo, in 30 patients with bilateral Parkinson's disease. Fifteen healthy control subjects also underwent neuromelanin-sensitive imaging. We used a novel approach taking into account the anatomical and functional subdivision of substantia nigra into dorsal and ventral tiers and striatal nuclei into pre- and post-commissural subregions, in accordance with previous animal and post-mortem studies, and consider the clinically asymmetric disease presentation. In vivo, Parkinson's disease subjects displayed reduced neuromelanin levels in the ventral (-30 ± 28%) and dorsal tiers (-21 ± 24%) as compared to the control group [F(1,43) = 11.95, P = 0.001]. Within the Parkinson's disease group, nigral pigmentation was lower in the ventral tier as compared to the dorsal tier [F(1,29) = 36.19, P < 0.001] and lower in the clinically-defined most affected side [F(1,29) = 4.85, P = 0.036]. Similarly, lower dopamine transporter density was observed in the ventral tier [F(1,29) = 76.39, P < 0.001] and clinically-defined most affected side [F(1,29) = 4.21, P = 0.049]. Despite similar patterns, regression analysis showed no significant association between nigral pigmentation and nigral dopamine transporter density. However, for the clinically-defined most affected side, significant relationships were observed between pigmentation of the ventral nigral tier with striatal dopamine transporter binding in pre-commissural and post-commissural striatal subregions known to receive nigrostriatal projections from this tier, while the dorsal tier correlated with striatal projection sites in the pre-commissural striatum (P < 0.05, Benjamini-Hochberg corrected). In contrast, there were no statistically significant relationships between these two measures in the clinically-defined least affected side. These findings provide important insights into the topography of nigrostriatal neurodegeneration in Parkinson's disease, indicating that the characteristics of disease progression may fundamentally differ across hemispheres and support post-mortem data showing asynchrony in the loss of neuromelanin-containing versus tyrosine hydroxylase positive nigral cells.

Citation

Martín-Bastida, A., Lao-Kaim, N. P., Roussakis, A. A., Searle, G. E., Xing, Y., Gunn, R. N., …Piccini, P. (2019). Relationship between neuromelanin and dopamine terminals within the Parkinson’s nigrostriatal system. Brain, 142(7), 2023–2036. https://doi.org/10.1093/brain/awz120

• Journal Article Type: Article
• Acceptance Date: Mar 5, 2019
• Online Publication Date: May 5, 2019
• Publication Date: Jul 1, 2019
• Deposit Date: May 29, 2019
• Publicly Available Date: May 6, 2020
• Journal: Brain
• Print ISSN: 0006-8950
• Electronic ISSN: 1460-2156
• Publisher: Oxford University Press
• Peer Reviewed: Peer Reviewed
• Volume: 142
• Issue: 7
• Pages: 2023–2036
• DOI: https://doi.org/10.1093/brain/awz120
• Public URL: https://nottingham-repository.worktribe.com/output/2104834
• Publisher URL: https://academic.oup.com/brain/advance-article/doi/10.1093/brain/awz120/5485828
• Additional Information: This is a pre-copyedited, author-produced version of an article accepted for publication in Brainfollowing peer review. The version of record Antonio Martín-Bastida, Nicholas P Lao-Kaim, Andreas Antonios Roussakis, Graham E Searle, Yue Xing, Roger N Gunn, Stefan T Schwarz, Roger A Barker, Dorothee P Auer, Paola Piccini, Relationship between neuromelanin and dopamine terminals within the Parkinson’s nigrostriatal system, Brain, , awz120 is available online at: https://doi.org/10.1093/brain/awz120.