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Quantum Sensing in a Physiological-Like Cell Niche Using Fluorescent Nanodiamonds Embedded in Electrospun Polymer Nanofibers

Price, Joshua C.; Levett, Simon J.; Radu, Valentin; Simpson, David A.; Barcons, Aina Mogas; Adams, Christopher F.; Mather, Melissa L.

Authors

Joshua C. Price

Simon J. Levett

Valentin Radu

David A. Simpson

Aina Mogas Barcons

Christopher F. Adams



Contributors

Abstract

© 2019 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim Fluorescent nanodiamonds (fNDs) containing nitrogen vacancy (NV) centers are promising candidates for quantum sensing in biological environments. This work describes the fabrication and implementation of electrospun poly lactic-co-glycolic acid (PLGA) nanofibers embedded with fNDs for optical quantum sensing in an environment, which recapitulates the nanoscale architecture and topography of the cell niche. A protocol that produces uniformly dispersed fNDs within electrospun nanofibers is demonstrated and the resulting fibers are characterized using fluorescent microscopy and scanning electron microscopy (SEM). Optically detected magnetic resonance (ODMR) and longitudinal spin relaxometry results for fNDs and embedded fNDs are compared. A new approach for fast detection of time varying magnetic fields external to the fND embedded nanofibers is demonstrated. ODMR spectra are successfully acquired from a culture of live differentiated neural stem cells functioning as a connected neural network grown on fND embedded nanofibers. This work advances the current state of the art in quantum sensing by providing a versatile sensing platform that can be tailored to produce physiological-like cell niches to replicate biologically relevant growth environments and fast measurement protocols for the detection of co-ordinated endogenous signals from clinically relevant populations of electrically active neuronal circuits.

Journal Article Type Article
Acceptance Date Apr 22, 2019
Online Publication Date Apr 22, 2019
Publication Date May 29, 2019
Deposit Date May 28, 2019
Journal Small
Print ISSN 1613-6810
Electronic ISSN 1613-6829
Publisher Wiley
Peer Reviewed Peer Reviewed
Volume 15
Issue 22
Article Number 1900455
DOI https://doi.org/10.1002/smll.201900455
Keywords Biotechnology; Engineering (miscellaneous); Biomaterials
Public URL https://nottingham-repository.worktribe.com/output/2102352
Publisher URL https://onlinelibrary.wiley.com/doi/full/10.1002/smll.201900455