Skip to main content

Research Repository

Advanced Search

Modelling the Effect of Intracellular Calcium in the Rundown of L-Type Calcium Current

Agrawal, Aditi; Clerx, Michael; Wang, Ken; Polonchuk, Liudmila; Gavaghan, David J.; Mirams, Gary R.

Modelling the Effect of Intracellular Calcium in the Rundown of L-Type Calcium Current Thumbnail


Authors

Aditi Agrawal

Ken Wang

Liudmila Polonchuk

David J. Gavaghan



Abstract

The L-type calcium current (ICaL) is a key current of the heart playing an important role in the contraction of the cardiomyocyte. Patch-clamp recordings of ionic currents can be associated with a reduction of the current magnitude with time (termed 'rundown'), and this phenomenon is particularly pronounced for ICaL recordings. Some part of rundown can be attributed to the unique sensitivity of L-type calcium channels (LCCs) to local [Ca2+]. In this paper, we study the experimental conditions in which rundown due to calcium-dependent inactivation (CDI) is minimised. We use first principles to derive an analytical equation for the diffusion of a calcium-chelating buffer from a patch hole on the cell's surface to the entirety of the cell. This determines the concentration profile of the buffer with respect to time and space. We then use the equation to simulate the effects of incoming calcium via the LCCs and its chemical reaction with the buffer.

Citation

Agrawal, A., Clerx, M., Wang, K., Polonchuk, L., Gavaghan, D. J., & Mirams, G. R. (2022, September). Modelling the Effect of Intracellular Calcium in the Rundown of L-Type Calcium Current. Presented at 2022 Computing in Cardiology Conference, Tampere, Finland

Presentation Conference Type Edited Proceedings
Conference Name 2022 Computing in Cardiology Conference
Start Date Sep 4, 2022
End Date Sep 7, 2022
Acceptance Date Jun 13, 2022
Online Publication Date Apr 3, 2023
Publication Date 2022
Deposit Date Oct 13, 2023
Publicly Available Date Nov 28, 2023
Publisher Institute of Electrical and Electronics Engineers
Peer Reviewed Peer Reviewed
Volume 2022-September
Pages 1044-1047
Series ISSN 2325-887X
Book Title 2022 Computing in Cardiology (CinC)
ISBN 9798350310139
DOI https://doi.org/10.22489/CinC.2022.051
Public URL https://nottingham-repository.worktribe.com/output/20008089
Publisher URL https://ieeexplore.ieee.org/document/10081742

Files

Modelling Rundown of L-Type Calcium Current (319 Kb)
PDF

Publisher Licence URL
https://creativecommons.org/licenses/by/4.0/

Copyright Statement
Articles in this Volume are Copyright © 2022 by their Respective Authors, and Licensed by their Authors under the Creative Commons Attribution 4.0 International License. (CCAL).





You might also like



Downloadable Citations