Analysis of the interaction and proliferative activity of adenocarcinoma, peripheral blood mononuclear and mesenchymal stromal cells after co-cultivation in vitro

Kitaeva, Kristina V.; Prudnikov, Tikhon S.; Gomzikova, Marina O.; Kletukhina, Sevindzh K.; James, Victoria; Rizvanov, Albert A.; Solovyeva, Valeriya V.

Abstract

The tumor microenvironment is a heterogeneous population of cells actively involved in the process of growth and development of a tumor. Research has demonstrated the interactions between the different populations of cells are critical for the formation of the tumor microenvironment and, if recapitulated experimentally, can be used to produce more effective models for preclinical screening of anticancer drugs. In this study, we demonstrate co-culturing HeLa adenocarcinoma cells, peripheral blood mononuclear cells, and mesenchymal stromal cells results in changes in the proliferative activity of the peripheral blood mononuclear cells and mesenchymal stromal cell populations. This data supports the further development of in vitro co-culture systems utilizing these cell types for pre-clinical screening of anticancer drugs.

Citation

Kitaeva, K. V., Prudnikov, T. S., Gomzikova, M. O., Kletukhina, S. K., James, V., Rizvanov, A. A., & Solovyeva, V. V. (2019). Analysis of the interaction and proliferative activity of adenocarcinoma, peripheral blood mononuclear and mesenchymal stromal cells after co-cultivation in vitro. BioNanoScience, 9(2), 502-509. https://doi.org/10.1007/s12668-019-00625-z

• Journal Article Type: Article
• Acceptance Date: Mar 15, 2019
• Online Publication Date: Mar 15, 2019
• Publication Date: Mar 15, 2019
• Deposit Date: Mar 29, 2019
• Publicly Available Date: Mar 16, 2020
• Journal: BioNanoScience
• Print ISSN: 2191-1630
• Electronic ISSN: 2191-1649
• Publisher: Springer Verlag
• Peer Reviewed: Peer Reviewed
• Volume: 9
• Issue: 2
• Pages: 502-509
• DOI: https://doi.org/10.1007/s12668-019-00625-z
• Keywords: Co-culture; Intercellular interaction; Adenocarcinoma; Mononuclear blood cell; Mesenchymal stromal cell; Test system; Drug screening
• Public URL: https://nottingham-repository.worktribe.com/output/1710270
• Publisher URL: https://link.springer.com/article/10.1007/s12668-019-00625-z
• Contract Date: Mar 29, 2019