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Self-association of the glycopeptide antibiotic teicoplanin A2 in aqueous solution studied by molecular hydrodynamics

Chun, Taewoo; Pattem, Jacob; Gillis, Richard B.; Dinu, Vlad T.; Yakubov, Gleb E.; Corfield, Anthony P.; Harding, Stephen E.


Taewoo Chun

Richard B. Gillis

Research Fellow

Anthony P. Corfield

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Professor of Applied Biochemistry


The natural glycopeptide antibiotic teicoplanin is used for the treatment of serious Gram-positive related bacterial infections and can be administered intravenously, intramuscularly, topically (ocular infections), or orally. It has also been considered for targeting viral infection by SARS-CoV-2. The hydrodynamic properties of teicoplanin A2 (M1 = 1880 g/mol) were examined in phosphate chloride buffer (pH 6.8, I = 0.10 M) using sedimentation velocity and sedimentation equilibrium in the analytical ultracentrifuge together with capillary (rolling ball) viscometry. In the concentration range, 0–10 mg/mL teicoplanin A2 was found to self-associate plateauing > 1 mg/mL to give a molar mass of (35,400 ± 1000) g/mol corresponding to ~ (19 ± 1) mers, with a sedimentation coefficient s20, w = ~ 4.65 S. The intrinsic viscosity [η] was found to be (3.2 ± 0.1) mL/g: both this, the value for s20,w and the hydrodynamic radius from dynamic light scattering are consistent with a globular macromolecular assembly, with a swelling ratio through dynamic hydration processes of ~ 2.


Chun, T., Pattem, J., Gillis, R. B., Dinu, V. T., Yakubov, G. E., Corfield, A. P., & Harding, S. E. (2023). Self-association of the glycopeptide antibiotic teicoplanin A2 in aqueous solution studied by molecular hydrodynamics. Scientific Reports, 13(1), Article 1969.

Journal Article Type Article
Acceptance Date Jan 24, 2023
Online Publication Date Feb 3, 2023
Publication Date Feb 3, 2023
Deposit Date Apr 15, 2023
Publicly Available Date Apr 18, 2023
Journal Scientific Reports
Electronic ISSN 2045-2322
Publisher Springer Science and Business Media LLC
Peer Reviewed Peer Reviewed
Volume 13
Issue 1
Article Number 1969
Public URL
Publisher URL
Additional Information Received: 31 October 2022; Accepted: 24 January 2023; First Online: 3 February 2023; : The authors declare no competing interests.


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