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A comparison of the mitochondrial proteome and lipidome in the mouse and long-lived Pipistrelle bats

Pollard, Amelia K.; Ingram, Thomas L.; Ortori, Catharine A.; Shephard, Freya; Brown, Margaret; Liddell, Susan; Barrett, David A.; Chakrabarti, Lisa

Authors

Amelia K. Pollard

Thomas L. Ingram

Catharine A. Ortori

Freya Shephard

Margaret Brown

Susan Liddell

David A. Barrett



Abstract

It is accepted that smaller mammals with higher metabolic rates have shorter lifespans. The very few species that do not follow these rules can give insights into interesting differences. The recorded maximum lifespans of bats are exceptional - over 40 years, compared with the laboratory mouse of 4 years. We investigated the differences in the biochemical composition of mitochondria between bat and mouse species. We used proteomics and ultra-high-performance liquid chromatography coupled with high resolution mass spectrometry lipidomics, to interrogate mitochondrial fractions prepared from Mus musculus and Pipistrellus pipistrellus brain and skeletal muscle. Fatty acid binding protein 3 was found at different levels in mouse and bat muscle mitochondria and its orthologues were investigated in Caenorhabditis elegans knock-downs for LBP 4, 5 and 6. In the bat, high levels of free fatty acids and N-acylethanolamine lipid species together with a significantly greater abundance of fatty acid binding protein 3 in muscle (1.8-fold, p=0.037) were found. Manipulation of fatty acid binding protein orthologues in C. elegans suggest these proteins and their role in lipid regulation are important for mitochondrial function.

Journal Article Type Article
Publication Date Mar 19, 2019
Journal Aging
Electronic ISSN 1945-4589
Publisher Impact Journals
Peer Reviewed Peer Reviewed
Volume 11
Issue 6
Pages 1664-1685
APA6 Citation Pollard, A. K., Ingram, T. L., Ortori, C. A., Shephard, F., Brown, M., Liddell, S., …Chakrabarti, L. (2019). A comparison of the mitochondrial proteome and lipidome in the mouse and long-lived Pipistrelle bats. Aging, 11(6), 1664-1685. https://doi.org/10.18632/aging.101861
DOI https://doi.org/10.18632/aging.101861
Publisher URL https://www.aging-us.com/article/101861/text
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