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Chloroplast/thylakoid-rich material: A possible alternative to the chemically synthesised flow enhancer polyglycerol polyricinoleate in oil-based systems

Sutcharit, Poramat; Wattanakul, Jutarat; Price, Ruth; Di Bari, Vincenzo; Gould, Joanne; Yakubov, Gleb; Wolf, Bettina; Gray, David A.

Chloroplast/thylakoid-rich material: A possible alternative to the chemically synthesised flow enhancer polyglycerol polyricinoleate in oil-based systems Thumbnail


Authors

Poramat Sutcharit

Jutarat Wattanakul

RUTH PRICE RUTH.PRICE1@NOTTINGHAM.AC.UK
Research Fellow

VINCENZO DI BARI Vincenzo.DiBari@nottingham.ac.uk
Assistant Professor in Food Structure

Bettina Wolf

DAVID GRAY david.gray@nottingham.ac.uk
Professor of Applied Lipid Science



Abstract

Chloroplasts are abundant organelles in a diverse range of plant materials; they are predominantly composed of multicomponent thylakoid membranes which are lipid and protein rich. Intact or unravelled thylakoid membranes should, in principle, have interfacial activity, but little has been published on their activity in oil-in-water systems, and nothing on their performance on an oil continuous system. In this work different physical methods were used to produce a range of chloroplast/thylakoid suspensions with varying degrees of membrane integrity. Transmission electron microscopy revealed that pressure homogenisation led to the greatest extent of membrane and organelle disruption compared to less energy intensive preparation methods The ability of the derived materials to modulate the flow behaviour of a chocolate model system (65% (w/w) sugar/ sunflower oil (natural amphiphiles removed) suspension) was investigated by acquiring rheological parameters. All chloroplast/thylakoid preparations reduced yield stress, apparent viscosity, tangent flow point and cross over point in a concentration-dependent fashion, although not as significantly as polyglycerol polyricinoleate applied at a commercially relevant concentration in the same chocolate model system. Confocal laser scanning microscopy confirmed presence of the alternative flow enhancer material at the sugar surfaces. This research reveals that low-energy processing methods that do not extensively disrupt thylakoid membranes are applicable to generating materials with marked capacity to affect the flow behaviour of a chocolate model system. In conclusion, chloroplast/thylakoid materials hold strong potential as natural alternatives to synthetic rheology modifiers for lipid-based systems such as PGPR.

Citation

Sutcharit, P., Wattanakul, J., Price, R., Di Bari, V., Gould, J., Yakubov, G., …Gray, D. A. (2023). Chloroplast/thylakoid-rich material: A possible alternative to the chemically synthesised flow enhancer polyglycerol polyricinoleate in oil-based systems. Food Research International, 165, Article 112472. https://doi.org/10.1016/j.foodres.2023.112472

Journal Article Type Article
Acceptance Date Jan 6, 2023
Online Publication Date Jan 9, 2023
Publication Date 2023-03
Deposit Date Jan 24, 2023
Publicly Available Date Mar 28, 2024
Journal Food Research International
Print ISSN 0963-9969
Electronic ISSN 1873-7145
Publisher Elsevier BV
Peer Reviewed Peer Reviewed
Volume 165
Article Number 112472
DOI https://doi.org/10.1016/j.foodres.2023.112472
Keywords Food Science
Public URL https://nottingham-repository.worktribe.com/output/16498128
Publisher URL https://www.sciencedirect.com/science/article/pii/S0963996923000170?via%3Dihub

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