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FT modulates genome-wide DNA-binding of the bZIP transcription factor FD

Collani, Silvio; Neumann, Manuela; Yant, Levi; Schmid, Markus

FT modulates genome-wide DNA-binding of the bZIP transcription factor FD Thumbnail


Authors

Silvio Collani

Manuela Neumann

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LEVI YANT LEVI.YANT@NOTTINGHAM.AC.UK
Professor of Evolutionary Genomics

Markus Schmid



Abstract

© 2019, American Society of Plant Biologists. All rights reserved. The transition to flowering is a crucial step in the plant life cycle that is controlled by multiple endogenous and environmental cues, including hormones, sugars, temperature, and photoperiod. Permissive photoperiod induces the expression of FLOWERING LOCUS T (FT) in the phloem companion cells of leaves. The FT protein then acts as a florigen that is transported to the shoot apical meristem, where it physically interacts with the Basic Leucine Zipper Domain transcription factor FD and 14-3-3 proteins. However, despite the importance of FD in promoting flowering, its direct transcriptional targets are largely unknown. Here, we combined chromatin immunoprecipitation sequencing and RNA sequencing to identify targets of FD at the genome scale and assessed the contribution of FT to DNA binding. We further investigated the ability of FD to form protein complexes with FT and TERMINAL FLOWER1 through interaction with 14-3-3 proteins. Importantly, we observed direct binding of FD to targets involved in several aspects of plant development. These target genes were previously unknown to be directly related to the regulation of flowering time. Our results confirm FD as a central regulator of floral transition at the shoot meristem and provide evidence for crosstalk between the regulation of flowering and other signaling pathways, such as pathways involved in hormone signaling.

Journal Article Type Article
Acceptance Date Feb 6, 2019
Online Publication Date Feb 15, 2019
Publication Date May 1, 2019
Deposit Date Feb 26, 2019
Publicly Available Date Feb 27, 2019
Journal Plant Physiology
Print ISSN 0032-0889
Electronic ISSN 1532-2548
Publisher American Society of Plant Biologists
Peer Reviewed Peer Reviewed
Volume 180
Issue 1
Pages 367-380
DOI https://doi.org/10.1104/pp.18.01505
Keywords Plant Science; Genetics; Physiology
Public URL https://nottingham-repository.worktribe.com/output/1586910

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