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Natural variation in a molybdate transporter controls grain molybdenum concentration in rice

Huang, Xin-Yuan; Liu, Huan; Zhu, Yu-Fei; Pinson, Shanon R M; Lin, Hong-Xuan; Guerinot, Mary Lou; Zhao, Fang-Jie; Salt, David E.

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Authors

Xin-Yuan Huang

Huan Liu

Yu-Fei Zhu

Shanon R M Pinson

Hong-Xuan Lin

Mary Lou Guerinot

Fang-Jie Zhao

DAVID SALT David.Salt@nottingham.ac.uk
Professor of Genome Enabled Biology



Abstract

© 2018 The Authors. New Phytologist © 2018 New Phytologist Trust Molybdenum (Mo) is an essential micronutrient for most living organisms, including humans. Cereals such as rice (Oryza sativa) are the major dietary source of Mo. However, little is known about the genetic basis of the variation in Mo content in rice grain. We mapped a quantitative trait locus (QTL) qGMo8 that controls Mo accumulation in rice grain by using a recombinant inbred line population and a backcross introgression line population. We identified a molybdate transporter, OsMOT1;1, as the causal gene for this QTL. OsMOT1;1 exhibits transport activity for molybdate, but not sulfate, when heterogeneously expressed in yeast cells. OsMOT1;1 is mainly expressed in roots and is involved in the uptake and translocation of molybdate under molybdate-limited condition. Knockdown of OsMOT1;1 results in less Mo being translocated to shoots, lower Mo concentration in grains and higher sensitivity to Mo deficiency. We reveal that the natural variation of Mo concentration in rice grains is attributed to the variable expression of OsMOT1;1 due to sequence variation in its promoter. Identification of natural allelic variation in OsMOT1;1 may facilitate the development of rice varieties with Mo-enriched grain for dietary needs and improve Mo nutrition of rice on Mo-deficient soils.

Citation

Huang, X., Liu, H., Zhu, Y., Pinson, S. R. M., Lin, H., Guerinot, M. L., …Salt, D. E. (2019). Natural variation in a molybdate transporter controls grain molybdenum concentration in rice. New Phytologist, 221(4), 1983-1997. https://doi.org/10.1111/nph.15546

Journal Article Type Article
Acceptance Date Oct 7, 2018
Online Publication Date Nov 23, 2018
Publication Date 2019-03
Deposit Date Jan 24, 2019
Publicly Available Date Nov 24, 2019
Journal New Phytologist
Print ISSN 0028-646X
Electronic ISSN 1469-8137
Publisher Wiley
Peer Reviewed Peer Reviewed
Volume 221
Issue 4
Pages 1983-1997
DOI https://doi.org/10.1111/nph.15546
Public URL https://nottingham-repository.worktribe.com/output/1494283
Publisher URL https://nph.onlinelibrary.wiley.com/doi/abs/10.1111/nph.15546
Additional Information This is the peer reviewed version of the following article: Huang, X. , Liu, H. , Zhu, Y. , Pinson, S. R., Lin, H. , Guerinot, M. L., Zhao, F. and Salt, D. E. (2018), Natural variation in a molybdate transporter controls grain molybdenum concentration in rice. New Phytol. , which has been published in final form at https://doi.org/10.1111/nph.15546. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.

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