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Impact of the partial replacement of fish meal with a chloroplast rich fraction on the growth and selected nutrient profile of zebrafish (Danio rerio)

Gedi, Mohamed A; Magee, Kieran; Darwish, Randa; Eakpetch, Patchaniya; Young, Iain; Gray, David

Authors

Mohamed A Gedi

Kieran Magee

Randa Darwish

Patchaniya Eakpetch

Iain Young

DAVID GRAY david.gray@nottingham.ac.uk
Professor of Applied Lipid Science



Abstract

In recent years increasing aquaculture production combined with a high variance in availability and cost of fishmeal and fish oil commodities, has led to a need to identify alternative source materials for protein and the polyunsaturated fatty acids (PUFAs) which fish oil is prized for containing. Chloroplasts are the organelles in plants’ leaves where many of the valuable nutrients, fatty acids (FAs), amino acids, vitamins and pigments, are synthesised. Chloroplasts could be incorporated into fish diets either retained in, or liberated from, plant cells. In this study zebrafish were fed with seven different diets individually; fish was fed with reducing fishmeal levels (10, 20 or 50%) by either spinach leaf powder (SLP) or a chloroplast rich fraction (CRF) prepared by an established method to recover chloroplasts. Both SLP and CRF had a positive impact on growth, taste response, whole fishFA composition, and carotenoid profile. Fish fed with CRF diets showed significantly (P≤0.05) greater α-linolenic (C18:3 n-3) and hexadecatrienoic (C16:3) acids content than those of SLP and the control. Hexadecanoic acid (C16:3) is a unique FA in the galactolipids of the chloroplast; its presence in zebrafish tissues proves that zebrafish digest and absorb chloroplast galactolipids. Lutein profile of eggs produced by zebrafish fed with CRF diet was significantly (P≤0.05) higher than that of SLP and control. Alterations in egg colour were also noted, warranting further investigations of diet impacts on fish fecundity, embryo fertility, hatch rate and larval survival.

Citation

Gedi, M. . A., Magee, K., Darwish, R., Eakpetch, P., Young, I., & Gray, D. (2019). Impact of the partial replacement of fish meal with a chloroplast rich fraction on the growth and selected nutrient profile of zebrafish (Danio rerio). Food and Function, 10(2), 733-745. https://doi.org/10.1039/c8fo02109k

Journal Article Type Article
Acceptance Date Jan 7, 2019
Online Publication Date Jan 15, 2019
Publication Date Jan 15, 2019
Deposit Date Jan 18, 2019
Publicly Available Date Jan 18, 2019
Journal Food & Function
Print ISSN 2042-6496
Electronic ISSN 2042-650X
Publisher Royal Society of Chemistry
Peer Reviewed Peer Reviewed
Volume 10
Issue 2
Pages 733-745
DOI https://doi.org/10.1039/c8fo02109k
Keywords Food Science; General Medicine
Public URL https://nottingham-repository.worktribe.com/output/1482083
Publisher URL https://pubs.rsc.org/en/Content/ArticleLanding/2019/FO/C8FO02109K