Impact of the partial replacement of fish meal with a chloroplast rich fraction on the growth and selected nutrient profile of zebrafish (Danio rerio)

Gedi, Mohamed A; Magee, Kieran; Darwish, Randa; Eakpetch, Patchaniya; Young, Iain; Gray, David

Abstract

In recent years increasing aquaculture production combined with a high variance in availability and cost of fishmeal and fish oil commodities, has led to a need to identify alternative source materials for protein and the polyunsaturated fatty acids (PUFAs) which fish oil is prized for containing. Chloroplasts are the organelles in plants’ leaves where many of the valuable nutrients, fatty acids (FAs), amino acids, vitamins and pigments, are synthesised. Chloroplasts could be incorporated into fish diets either retained in, or liberated from, plant cells. In this study zebrafish were fed with seven different diets individually; fish was fed with reducing fishmeal levels (10, 20 or 50%) by either spinach leaf powder (SLP) or a chloroplast rich fraction (CRF) prepared by an established method to recover chloroplasts. Both SLP and CRF had a positive impact on growth, taste response, whole fishFA composition, and carotenoid profile. Fish fed with CRF diets showed significantly (P≤0.05) greater α-linolenic (C18:3 n-3) and hexadecatrienoic (C16:3) acids content than those of SLP and the control. Hexadecanoic acid (C16:3) is a unique FA in the galactolipids of the chloroplast; its presence in zebrafish tissues proves that zebrafish digest and absorb chloroplast galactolipids. Lutein profile of eggs produced by zebrafish fed with CRF diet was significantly (P≤0.05) higher than that of SLP and control. Alterations in egg colour were also noted, warranting further investigations of diet impacts on fish fecundity, embryo fertility, hatch rate and larval survival.

Citation

Gedi, M. . A., Magee, K., Darwish, R., Eakpetch, P., Young, I., & Gray, D. (2019). Impact of the partial replacement of fish meal with a chloroplast rich fraction on the growth and selected nutrient profile of zebrafish (Danio rerio). Food and Function, 10(2), 733-745. https://doi.org/10.1039/c8fo02109k

• Journal Article Type: Article
• Acceptance Date: Jan 7, 2019
• Online Publication Date: Jan 15, 2019
• Publication Date: Jan 15, 2019
• Deposit Date: Jan 18, 2019
• Publicly Available Date: Jan 18, 2019
• Journal: Food & Function
• Print ISSN: 2042-6496
• Electronic ISSN: 2042-650X
• Publisher: Royal Society of Chemistry
• Peer Reviewed: Peer Reviewed
• Volume: 10
• Issue: 2
• Pages: 733-745
• DOI: https://doi.org/10.1039/c8fo02109k
• Keywords: Food Science; General Medicine
• Public URL: https://nottingham-repository.worktribe.com/output/1482083
• Publisher URL: https://pubs.rsc.org/en/Content/ArticleLanding/2019/FO/C8FO02109K
• Contract Date: Jan 18, 2019