Benita C. Percival
Low-field, benchtop NMR spectroscopy as a potential tool for point-of-care diagnostics of metabolic conditions: validation, protocols and computational models
Percival, Benita C.; Grootveld, Martin; Gibson, Miles; Osman, Yasan; Molinari, Marco; Jafari, Fereshteh; Sahota, Tarsem; Martin, Mark; Casanova, Federico; Mather, Melissa L.; Edgar, Mark; Masania, Jinit; Wilson, Philippe B.
Melissa L. Mather
Philippe B. Wilson
Novel sensing technologies for liquid biopsies offer promising prospects for the early detection of metabolic conditions through omics techniques. Indeed, high-field nuclear magnetic resonance (NMR) facilities are routinely used for metabolomics investigations on a range of biofluids in order to rapidly recognise unusual metabolic patterns in patients suffering from a range of diseases. However, these techniques are restricted by the prohibitively large size and cost of such facilities, suggesting a possible role for smaller, low-field NMR instruments in biofluid analysis. Herein we describe selected biomolecule validation on a low-field benchtop NMR spectrometer (60 MHz), and present an associated protocol for the analysis of biofluids on compact NMR instruments. We successfully detect common markers of diabetic control at low-to-medium concentrations through optimised experiments, including α-glucose (≤2.8 mmol/L) and acetone (25 µmol/L), and additionally in readily accessible biofluids, particularly human urine. We present a combined protocol for the analysis of these biofluids with low-field NMR spectrometers for metabolomics applications, and offer a perspective on the future of this technique appealing to ‘point-of-care’ applications.
|Journal Article Type||Article|
|Publication Date||Jan 4, 2019|
|Peer Reviewed||Peer Reviewed|
|APA6 Citation||Percival, B. C., Grootveld, M., Gibson, M., Osman, Y., Molinari, M., Jafari, F., …Wilson, P. B. (2019). Low-field, benchtop NMR spectroscopy as a potential tool for point-of-care diagnostics of metabolic conditions: validation, protocols and computational models. High-Throughput, 8(1), doi:10.3390/ht8010002|
Low-Field, Benchtop NMR Spectroscopy
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