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Low-field, benchtop NMR spectroscopy as a potential tool for point-of-care diagnostics of metabolic conditions: validation, protocols and computational models

Percival, Benita C.; Grootveld, Martin; Gibson, Miles; Osman, Yasan; Molinari, Marco; Jafari, Fereshteh; Sahota, Tarsem; Martin, Mark; Casanova, Federico; Mather, Melissa L.; Edgar, Mark; Masania, Jinit; Wilson, Philippe B.

Authors

Benita C. Percival

Martin Grootveld

Miles Gibson

Yasan Osman

Marco Molinari

Fereshteh Jafari

Tarsem Sahota

Mark Martin

Federico Casanova

Melissa L. Mather

Mark Edgar

Jinit Masania

Philippe B. Wilson



Abstract

Novel sensing technologies for liquid biopsies offer promising prospects for the early detection of metabolic conditions through omics techniques. Indeed, high-field nuclear magnetic resonance (NMR) facilities are routinely used for metabolomics investigations on a range of biofluids in order to rapidly recognise unusual metabolic patterns in patients suffering from a range of diseases. However, these techniques are restricted by the prohibitively large size and cost of such facilities, suggesting a possible role for smaller, low-field NMR instruments in biofluid analysis. Herein we describe selected biomolecule validation on a low-field benchtop NMR spectrometer (60 MHz), and present an associated protocol for the analysis of biofluids on compact NMR instruments. We successfully detect common markers of diabetic control at low-to-medium concentrations through optimised experiments, including α-glucose (≤2.8 mmol/L) and acetone (25 µmol/L), and additionally in readily accessible biofluids, particularly human urine. We present a combined protocol for the analysis of these biofluids with low-field NMR spectrometers for metabolomics applications, and offer a perspective on the future of this technique appealing to ‘point-of-care’ applications.

Journal Article Type Article
Publication Date Jan 4, 2019
Journal High-Throughput
Print ISSN 2571-5135
Publisher MDPI
Peer Reviewed Peer Reviewed
Volume 8
Issue 1
Article Number 2
APA6 Citation Percival, B. C., Grootveld, M., Gibson, M., Osman, Y., Molinari, M., Jafari, F., …Wilson, P. B. (2019). Low-field, benchtop NMR spectroscopy as a potential tool for point-of-care diagnostics of metabolic conditions: validation, protocols and computational models. High-Throughput, 8(1), doi:10.3390/ht8010002
DOI https://doi.org/10.3390/ht8010002
Publisher URL https://www.mdpi.com/2571-5135/8/1/2

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