Benita C. Percival
Low-field, benchtop NMR spectroscopy as a potential tool for point-of-care diagnostics of metabolic conditions: Validation, protocols and computational models
Percival, Benita C.; Grootveld, Martin; Gibson, Miles; Osman, Yasan; Molinari, Marco; Jafari, Fereshteh; Sahota, Tarsem; Martin, Mark; Casanova, Federico; Mather, Melissa L.; Edgar, Mark; Masania, Jinit; Wilson, Philippe B.
Authors
Martin Grootveld
Miles Gibson
Yasan Osman
Marco Molinari
Fereshteh Jafari
Tarsem Sahota
Mark Martin
Federico Casanova
Professor MELISSA MATHER Melissa.Mather@nottingham.ac.uk
Professor of Quantum Sensing and Engineering
Mark Edgar
Jinit Masania
Philippe B. Wilson
Abstract
Novel sensing technologies for liquid biopsies offer promising prospects for the early detection of metabolic conditions through omics techniques. Indeed, high-field nuclear magnetic resonance (NMR) facilities are routinely used for metabolomics investigations on a range of biofluids in order to rapidly recognise unusual metabolic patterns in patients suffering from a range of diseases. However, these techniques are restricted by the prohibitively large size and cost of such facilities, suggesting a possible role for smaller, low-field NMR instruments in biofluid analysis. Herein we describe selected biomolecule validation on a low-field benchtop NMR spectrometer (60 MHz), and present an associated protocol for the analysis of biofluids on compact NMR instruments. We successfully detect common markers of diabetic control at low-to-medium concentrations through optimised experiments, including α-glucose (≤2.8 mmol/L) and acetone (25 µmol/L), and additionally in readily accessible biofluids, particularly human urine. We present a combined protocol for the analysis of these biofluids with low-field NMR spectrometers for metabolomics applications, and offer a perspective on the future of this technique appealing to ‘point-of-care’ applications.
Citation
Percival, B. C., Grootveld, M., Gibson, M., Osman, Y., Molinari, M., Jafari, F., …Wilson, P. B. (2019). Low-field, benchtop NMR spectroscopy as a potential tool for point-of-care diagnostics of metabolic conditions: Validation, protocols and computational models. High-Throughput, 8(1), Article 2. https://doi.org/10.3390/ht8010002
Journal Article Type | Article |
---|---|
Acceptance Date | Dec 13, 2018 |
Online Publication Date | Dec 27, 2018 |
Publication Date | Jan 1, 2019 |
Deposit Date | Jan 10, 2019 |
Publicly Available Date | Jan 11, 2019 |
Journal | High-Throughput |
Print ISSN | 2571-5135 |
Publisher | MDPI |
Peer Reviewed | Peer Reviewed |
Volume | 8 |
Issue | 1 |
Article Number | 2 |
DOI | https://doi.org/10.3390/ht8010002 |
Public URL | https://nottingham-repository.worktribe.com/output/1463138 |
Publisher URL | https://www.mdpi.com/2571-5135/8/1/2 |
Contract Date | Jan 11, 2019 |
Files
Low-Field, Benchtop NMR Spectroscopy
(3.4 Mb)
PDF
Publisher Licence URL
https://creativecommons.org/licenses/by/4.0/
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