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Tapping into synchrotron and benchtop circular dichroism spectroscopy for expanding studies of complex polysaccharides and their interactions in anoxic archaeological wood

Phillips-Jones, Mary K.; Harding, Stephen E.

Tapping into synchrotron and benchtop circular dichroism spectroscopy for expanding studies of complex polysaccharides and their interactions in anoxic archaeological wood Thumbnail


Authors

Mary K. Phillips-Jones

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STEPHEN HARDING STEVE.HARDING@NOTTINGHAM.AC.UK
Professor of Applied Biochemistry



Abstract

Circular dichroism (CD) (and synchrotron circular dichroism (SCD)) spectroscopy is a rapid, highly sensitive technique used to investigate structural conformational changes in biomolecules in response to interactions with ligands in solution and in film. It is a chiroptical method and at least one of the interacting molecules must possess optical activity (or chirality). In this review, we compare the capabilities of CD and SCD in the characterisation of celluloses and lignin polymers in archaeological wood. Cellulose produces a range of spectral characteristics dependent on environment and form; many of the reported transitions occur in the vacuum-ultraviolet region (< 180 nm) most conveniently delivered using a synchrotron source. The use of induced CD in which achiral dyes are bound to celluloses to give shifted spectra in the visible region is also discussed, together with its employment to identify the handedness of the chiral twists in nanocrystalline cellulose. Lignin is one target for the design of future consolidants that interact with archaeological wood to preserve it. It is reportedly achiral, but here we review several studies in which CD spectroscopy has successfully revealed lignin interactions with chiral enzymes, highlighting the potential usefulness of the technique in future research to identify new generation consolidants.

Journal Article Type Article
Acceptance Date Jan 5, 2019
Online Publication Date Jan 9, 2019
Publication Date Mar 1, 2019
Deposit Date Jan 10, 2019
Publicly Available Date Jan 10, 2019
Journal Heritage
Print ISSN 2571-9408
Electronic ISSN 2571-9408
Publisher MDPI
Peer Reviewed Peer Reviewed
Volume 2
Issue 1
Pages 121-134
DOI https://doi.org/10.3390/heritage2010009
Public URL https://nottingham-repository.worktribe.com/output/1461618
Publisher URL https://www.mdpi.com/2571-9408/2/1/9

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